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The so\u2010called high\u2010resolution RNA structural Fragment Assembly of RNA with Full\u2010Atom Refinement 2 (FARFAR2) ensembles of the conserved 5\n                    <jats:sup>\u2032<\/jats:sup>\n                    \u2010terminal stem\u2010loop 1 (SL1) and the pseudoknot of the \u20131 programed frameshift element are used as targets for binding of small molecules of three different virtual libraries of compounds. The resulting hits predicted by virtual screening are probed for their binding to the two RNA elements by ligand\u2010 and target\u2010based NMR experiments. The results demonstrate that the integration of virtual and experimental NMR screening efficiently identifies RNA\u2010binding small molecules as start molecular entities to advance RNA\u2010targeted antiviral therapies in an efficient manner. The strategy does not only apply to SARS\u2010CoV\u20102, but also provides a rapid, highly specific route to discovering therapeutics for other RNA\u2010based pathogens, highlighting the critical role of RNA structural data in enriching virtual drug discovery efforts.\n                  <\/jats:p>","DOI":"10.1002\/cbic.202500668","type":"journal-article","created":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T13:19:39Z","timestamp":1764422379000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Integrative In\u2009Silico and In\u2009Vitro Screening of Low Molecular Weight Compounds Targeting SARS\u2010CoV\u20102 RNA Elements"],"prefix":"10.1002","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-5501-6276","authenticated-orcid":false,"given":"Sabrina","family":"Toews","sequence":"first","affiliation":[{"name":"Institute for Organic Chemistry and Chemical Biology Goethe University Frankfurt  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