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Formate can serve as both a carbon and energy source, further enhancing its attractiveness as a feedstock.\n                    <jats:italic>Cupriavidus necator<\/jats:italic>\n                    , a lithoautotrophic microbial chassis strain, provides an opportunity to leverage formate for the synthesis of valuable products. However, its ability to grow on formate and the subsequent coupling of that process to recombinantly produced redox enzymes for the efficient production of high\u2010value\u2010added products in a biotransformation has not yet been established. Here, we report the development of a formate\u2010driven\n                    <jats:italic>C. necator<\/jats:italic>\n                    whole\u2010cell chassis that recombinantly produces Rieske oxygenases (ROs) and elaborate on possible stress responses of the cells during formatotrophic cultivation. The whole\u2010cell chassis efficiently catalyzes the oxyfunctionalization of olefins fueled by formate oxidation. For instance, styrene is dihydroxylated to (\n                    <jats:italic>R<\/jats:italic>\n                    )\u20101\u2010phenylethane\u20101,2\u2010diol in an excellent 95% yield and with good enantioselectivity (74%\n                    <jats:italic>ee<\/jats:italic>\n                    ) under formatotrophic conditions. The product yield and optical purity obtained demonstrate the synthetic usefulness of formate\u2010fueled whole\u2010cell bio\u2010transformations in\n                    <jats:italic>C. necator<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/cbic.202500722","type":"journal-article","created":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T09:44:31Z","timestamp":1760003071000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Rieske Oxygenase\u2010Catalyzed Biotransformations in Recombinant\n                    <i>Cupriavidus necator<\/i>\n                    Fueled by Formate Oxidation"],"prefix":"10.1002","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3915-911X","authenticated-orcid":false,"given":"Marleen","family":"Hallamaa","sequence":"first","affiliation":[{"name":"Department of Chemical and Pharmaceutical Biology Groningen Research Institute of Pharmacy University of Groningen  Antonius Deusinglaan 1 9713 AV Groningen Netherlands"},{"name":"Faculty of Science Department of Chemistry University of Helsinki  A. 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Virtasen aukio 1 00560 Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah Pia Franziska","family":"Meier","sequence":"additional","affiliation":[{"name":"Department of Chemical and Pharmaceutical Biology Groningen Research Institute of Pharmacy University of Groningen  Antonius Deusinglaan 1 9713 AV Groningen Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8589-9072","authenticated-orcid":false,"given":"Matteo","family":"Vajente","sequence":"additional","affiliation":[{"name":"Department of Chemical and Pharmaceutical Biology Groningen Research Institute of Pharmacy University of Groningen  Antonius Deusinglaan 1 9713 AV Groningen Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mattia","family":"Ghirardi","sequence":"additional","affiliation":[{"name":"Department of Chemical and Pharmaceutical Biology Groningen Research Institute of Pharmacy University of Groningen  Antonius Deusinglaan 1 9713 AV Groningen Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Deska","sequence":"additional","affiliation":[{"name":"Faculty of Science Department of Chemistry University of Helsinki  A. 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