{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T11:04:35Z","timestamp":1770980675729,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T00:00:00Z","timestamp":1770854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T00:00:00Z","timestamp":1770854400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2026,2,12]]},"abstract":"<jats:p>\n                    Cell transplantation therapy is a promising strategy for next\u2010generation regenerative medicine. However, its clinical application is limited by the absence of safe and label\u2010free methods for obtaining pure populations of functional cells. This study reports a peptide\u2010functionalized zwitterionic polymer system designed for the selective and noninvasive isolation of human mesenchymal stem cells (hMSCs) from heterogeneous cell populations. The substrate was modified with poly(CMBMAm\u2010\n                    <jats:italic>co<\/jats:italic>\n                    \u2010PGMAn\u2010\n                    <jats:italic>co<\/jats:italic>\n                    \u2010MPTMS1) (PC\n                    <jats:italic>\n                      <jats:sub>m<\/jats:sub>\n                    <\/jats:italic>\n                    P\n                    <jats:sub>\n                      <jats:italic>n<\/jats:italic>\n                    <\/jats:sub>\n                    M\n                    <jats:sub>1<\/jats:sub>\n                    ), which was synthesized via free radical polymerization, and subsequently conjugated with a CD44\u2010binding peptide (CD44BP). Among the compositions examined, the PC\n                    <jats:sub>7<\/jats:sub>\n                    P\n                    <jats:sub>2<\/jats:sub>\n                    M\n                    <jats:sub>1<\/jats:sub>\n                    \u2013CD44BP surface exhibited superior antifouling properties, effectively suppressing nonspecific protein adsorption and adhesion of nontarget cells while selectively capturing hMSCs. A column packed with PC\n                    <jats:sub>7<\/jats:sub>\n                    P\n                    <jats:sub>2<\/jats:sub>\n                    M\n                    <jats:sub>1<\/jats:sub>\n                    \u2013CD44BP\u2013modified silica microparticles successfully isolated high\u2010purity hMSCs from mixed cell suspensions within 35\u2009min. Flow cytometry confirmed that the cells eluted later exhibited higher CD44 and CD105 expression, indicating separation based on antigen expression. The isolated hMSCs maintained proliferation and differentiation capacities equivalent to those of the preseparated cells, demonstrating that this device preserved cell functionality. This peptide\u2013polymer hybrid column provides a simple and clinically adaptable platform for safe, label\u2010free purification of hMSCs intended for cell transplantation therapy.\n                  <\/jats:p>","DOI":"10.1002\/cbic.202500822","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T09:03:47Z","timestamp":1770887027000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["CD44\u2010Binding Peptide\u2010Functionalized Antibiofouling Polymer Surface for High\u2010Performance Separation of Human Mesenchymal Stromal Cells"],"prefix":"10.1002","volume":"27","author":[{"given":"Moe","family":"Kato","sequence":"first","affiliation":[{"name":"Graduate School of Innovative Life Science University of Toyama  Toyama 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