{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T07:12:18Z","timestamp":1788246738492,"version":"build-2803163510"},"reference-count":50,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:00:00Z","timestamp":1776902400000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:p>\n                    Conventional single\u2010stranded antisense oligonucleotides (ASOs) work by directly binding target mRNA, which limits their use to genes that need to be silenced without affecting healthy cells. To address this limitation and achieve conditional gene silencing specifically within target cells, we developed Antisense Oligonucleotide Releasing Cassette (ARC). This system activates its RNA\u2010cleaving function only upon binding to a specific biomarker nucleic acid sequence followed by RNase H\u2010dependent cleavage of the targeted RNA. Under cell\u2010free conditions, ARC showed 3\u2010fold greater RNA cleavage efficiency in the presence of the biomarker as compared to its absence. Intriguingly, in the ARC\u2010treated\n                    <jats:italic>GFP<\/jats:italic>\n                    \u2010expressing K562 cell line a 45% reduction in\n                    <jats:italic>GFP<\/jats:italic>\n                    fluorescence was achieved in the presence of the biomarker sequence, which was 5.3\u2010fold greater than the reduction observed when it was absent. This ARC's conditional ASO release offers a new mechanism for safely targeting essential or vital genes exclusively in cells that produce specific RNA biomarkers. Our findings indicate that the future in the field of nucleic acid nanotechnology will critically depend on two key advancements: the development of appropriate intracellular delivery systems and the establishment of robust predictive tools for accurately modeling the thermodynamic behavior of complex nanostructures, including those incorporating diverse chemical modifications.\n                  <\/jats:p>","DOI":"10.1002\/cbic.202500899","type":"journal-article","created":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T14:32:59Z","timestamp":1776954779000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Antisense Oligonucleotide Releasing Cassette for Conditional Targeted RNA Degradation"],"prefix":"10.1002","volume":"27","author":[{"given":"Valeriia S.","family":"Drozd","sequence":"first","affiliation":[{"name":"Nucleic acid nanotechnology laboratory ITMO University  Saint\u2010Petersburg Russian Federation"},{"name":"Advanced engineering school ITMO University  Almetyvsk Russian Federation"},{"name":"Belozersky Institute of Physico\u2010Chemical Biology Lomonosov Moscow State University  Moscow Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad S.","family":"Anwar","sequence":"additional","affiliation":[{"name":"Nucleic acid nanotechnology laboratory ITMO University  Saint\u2010Petersburg Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adeliia A.","family":"Salimova","sequence":"additional","affiliation":[{"name":"Nucleic acid nanotechnology laboratory ITMO University  Saint\u2010Petersburg Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daria S.","family":"Rybalko","sequence":"additional","affiliation":[{"name":"Nucleic acid nanotechnology laboratory ITMO University  Saint\u2010Petersburg Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana V.","family":"Vasileva","sequence":"additional","affiliation":[{"name":"Nucleic acid nanotechnology laboratory ITMO University  Saint\u2010Petersburg Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor V.","family":"Tatarskiy","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Oncobiology Institute of Gene Biology of the Russian Academy of Sciences  Moscow Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry M.","family":"Kolpashchikov","sequence":"additional","affiliation":[{"name":"Chemistry department University of Central Florida  Orlando USA"},{"name":"Burnett School of Biomedical Sciences University of Central Florida  Orlando USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed A.","family":"Eldeeb","sequence":"additional","affiliation":[{"name":"Nucleic acid nanotechnology laboratory ITMO University  Saint\u2010Petersburg Russian Federation"},{"name":"Advanced engineering school ITMO University  Almetyvsk Russian Federation"},{"name":"Belozersky Institute of Physico\u2010Chemical Biology Lomonosov Moscow State University  Moscow Russian Federation"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201900829"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.77830"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/nat.2023.0027"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2023.29263.bfs"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2025.02.045"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201900525"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gng077"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0097764"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja404676x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja905596t"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2021.12.039"},{"key":"e_1_2_7_13_1","unstructured":"S.Han L.Scherer A. M.Salvador\u2010Garicano et al. \u201cDevelopment and Optimization of Logic Gated Small Interfering RNAs for Operation Inside Mammalian Cells \u201d in23rd Annual Meeting of the American Society for Gene and Cell Therapy (2020):28."},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2009.03.016"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.6b05628"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3AN02178E"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202400665"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-38100-9"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.1c00387"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1089\/nat.2021.0108"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4CC00495G"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3765"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.3c05144"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4CS00799A"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2008.03.012"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2014.154"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13061204"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv992"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2005.12.018"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr900323b"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae295"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202508122"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2024.155161"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15092414"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2020.03.003"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/9513037"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.09.044"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv964"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2RA04375K"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.2011402"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60327-429-6_1"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21596"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1089\/nat.2021.0055"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M009676200"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1089\/nat.2019.0804"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.079507.122"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2612"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.3109\/10409238.2012.694844"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41421-020-0167-x"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1080\/10611860310001636908"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2020.01.018"}],"container-title":["ChemBioChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.202500899","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbic.202500899","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.202500899","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T05:58:35Z","timestamp":1777615115000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cbic.202500899"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":50,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/cbic.202500899"],"URL":"https:\/\/doi.org\/10.1002\/cbic.202500899","archive":["Portico"],"relation":{},"ISSN":["1439-4227","1439-7633"],"issn-type":[{"value":"1439-4227","type":"print"},{"value":"1439-7633","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"2025-11-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202500899"}}