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In this study, the interfacial and biological consequences of bovine serum albumin (BSA) adsorption on two silicate BG compositions, 45S5 and 59S, are systematically evaluated. Protein adsorption markedly alters the surface\u2010mediated bioactivity of both glasses, with 59S BG exhibiting significantly greater protein affinity, consistent with its compositional characteristics and surface reactivity. Protein\u2010conditioned BGs demonstrate enhanced cytocompatibility, supporting improved fibroblast adhesion, and proliferation, with the most pronounced cellular response observed for 59S BG\/BSA. Ex vivo angiogenesis assays further reveal a substantial enhancement in vascular network formation following protein conditioning, following the trend: Control\u2009&lt;\u200945S5 BG\u2009&lt;\u200945S5 BG\/BSA\u2009&lt;\u200959S BG\u2009&lt;\u200959S BG\/BSA. These effects are attributed to the synergistic interaction between sustained therapeutic ion release and the establishment of a biologically active protein\u2010modified interface. Collectively, the results show that protein adsorption fundamentally regulates BG\u2010biological interactions and significantly enhances angiogenic and cell\u2010supportive responses, positioning protein\u2010conditioned 59S BG as a robust candidate for advanced tissue regeneration applications.<\/jats:p>","DOI":"10.1002\/cbic.202500940","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:42:51Z","timestamp":1772991771000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Interfacial Protein Layer Formation on 45S5 and 59S Bioactive Glasses Enhances Cellular Compatibility and Angiogenic Activity"],"prefix":"10.1002","volume":"27","author":[{"given":"Elakkiya","family":"Krishnamoorthy","sequence":"first","affiliation":[{"name":"National Centre for Nanoscience and Nanotechnology University of Madras  Chennai TamilNadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7764-5865","authenticated-orcid":false,"given":"Balakumar","family":"Subramanian","sequence":"additional","affiliation":[{"name":"National Centre for Nanoscience and Nanotechnology University of Madras  Chennai TamilNadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,25]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma14185440"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.5312\/wjo.v15.i3.204"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apt.2019.06.002"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0RA04287K"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oceram.2025.100747"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3SC06549A"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/jfb15090258"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano11030561"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/slct.202304337"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.1c21378"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.4c08487"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.1c01322"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2024.125026"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.35159"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13758-012-0041-y"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4RA06081D"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/rb\/rbae110"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2012.04.008"},{"key":"e_1_2_10_20_1","unstructured":"G. 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