{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T07:24:09Z","timestamp":1776237849618,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2026,4,14]]},"abstract":"<jats:p>\n                    We introduce droplet microfluidics\u2010fabricated hybrid microgels composed of biotinylated acrylamide that is crosslinked with\n                    <jats:italic>N<\/jats:italic>\n                    ,\n                    <jats:italic>N<\/jats:italic>\n                    \u2019\u2010methylenebis(acrylamide). These microgels are further functionalized with streptavidin and loaded with magnetite nanoparticles (MNPs). During microfluidic processing, MNPs remain dispersed in an aqueous methylcellulose solution for over 4\u2009h due to increased viscous drag, enabling stable droplet formation. In contrast, the MNPs sediment within a few minutes in pure aqueous solution. The resulting multifunctional hybrid microgels facilitate straightforward protein immobilization under mild conditions, for example, utilizing enzymes conjugated with streptavidin or recombinant fusion proteins of magnetite\u2010binding proteins. Simultaneously, our microgels enable the recovery of immobilized proteins through magnetic separation of the microgels from solution. The quantity of immobilized proteins can be regulated independently by varying the amount of coupled biotin or encapsulated MNPs. Using microgels containing different quantities of coupled biotin, we demonstrate binding of a streptavidin\u2010conjugated fluorescent dye and horseradish peroxidase. To confirm the availability of MNPs for magnetite\u2010binding proteins, a fusion protein of the magnetite\u2010binding protein Mad10 and super\u2010folder green fluorescent protein (sfGFP) was immobilized, and its fluorescence was detected.\n                  <\/jats:p>","DOI":"10.1002\/cbic.202500958","type":"journal-article","created":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T05:38:15Z","timestamp":1775021895000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Droplet Microfluidics\u2010Assisted Fabrication of Magnetite Nanoparticle Hybrid Microgels for Facile Protein Immobilization"],"prefix":"10.1002","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2335-3637","authenticated-orcid":false,"given":"Talika A.","family":"Neuendorf","sequence":"first","affiliation":[{"name":"Institute of Physical Chemistry and Polymer Physics Leibniz Institute of Polymer Research Dresden  Dresden Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6877-4316","authenticated-orcid":false,"given":"Anika","family":"Kaufmann","sequence":"additional","affiliation":[{"name":"Institute of Physical Chemistry and Polymer Physics Leibniz Institute of Polymer Research Dresden  Dresden Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6507-2089","authenticated-orcid":false,"given":"Russell J.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Department of Biomolecular and Self-Organizing Matter Institute of Experimental Physics Johannes Kepler University Linz  Linz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5410-6984","authenticated-orcid":false,"given":"Kerstin G.","family":"Blank","sequence":"additional","affiliation":[{"name":"Department of Biomolecular and Self-Organizing Matter Institute of Experimental Physics Johannes Kepler University Linz  Linz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5449-3048","authenticated-orcid":false,"given":"Julian","family":"Thiele","sequence":"additional","affiliation":[{"name":"Institute of Physical Chemistry and Polymer Physics Leibniz Institute of Polymer Research Dresden  Dresden Germany"},{"name":"Institute of Chemistry Otto von Guericke University Magdeburg  Magdeburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcatb.2008.12.001"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2015.09.100"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.iecr.0c03281"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2021.134128"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4RA02939A"},{"key":"e_1_2_9_7_1","first-page":"109","volume-title":"Nanoparticle Technology Handbook","author":"Naito M.","year":"2018"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules18077533"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.8b04304"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matlet.2007.07.011"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/la040084f"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cep.2017.03.009"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/s23125399"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja209245v"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7NR07539A"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiec.2010.09.001"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.200900499"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3109\/17435390.2010.528846"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3281"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/gels8020117"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.progpolymsci.2020.101243"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(90)90328-S"},{"key":"e_1_2_9_23_1","first-page":"85","volume-title":"Advances in Protein Chemistry","author":"Green N. 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