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For optimal immune activation with viral antigens, we applied covalent antigen\u2013adjuvant complexation at the molecular level, which enables refined modulation of immune responses. This antigen\u2010complex strategy was applied to viral peptide antigens containing sequence regions with lower variability, a particularly valuable approach for rapidly mutating viruses such as influenza. To construct these conjugated antigen complex structures, we used\n                    <jats:italic>\u03b1<\/jats:italic>\n                    \u2010GalCer as the lipid antigen adjuvant and optimized linker designs that markedly influenced immunomodulatory activity. Using the precisely synthesized antigen complexes along with human leukocyte antigen (HLA)\u2010transgenic mice, we successfully demonstrated selective immune activation, particularly the peptide antigen\u2010specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell expansion. These methods can\u00a0 be extended to other viral antigens and may facilitate the development of CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell\u2010based self\u2010adjuvanting conjugate vaccines.\n                  <\/jats:p>","DOI":"10.1002\/cbic.202500979","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T20:30:13Z","timestamp":1773001813000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Development of a Self\u2010Adjuvanting Influenza Peptide\u2013Glycolipid Conjugate Inducing CD8\n                    <sup>+<\/sup>\n                    T\u2010Cell Immunity"],"prefix":"10.1002","volume":"27","author":[{"given":"Shunya","family":"Kikuchi","sequence":"first","affiliation":[{"name":"Department of Chemistry Faculty of Science and Technology Keio University  Yokohama Japan"},{"name":"Human Biology Microbiome Quantum Research Center (Bio2Q) Keio 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