{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T10:41:41Z","timestamp":1777891301951,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:p>Fluorescent probes have been widely developed for detecting amyloid biomarkers associated with neurodegenerative diseases (NDs). The requirement for external light for excitation, however, limits their utility for research and in vivo applications. Chemiluminescent probes, which use chemical reactions to generate emissive excited states rather than externally applied light, may offer a promising alternative to overcome some limitations for optical imaging of amyloid biomarkers. Here, we designed and synthesized a chemiluminescent amyloid\u2010binding probe (CLIP\u20101) and evaluated its capability to label three amyloid biomarkers for NDs\u2014amyloid \u03b2 (A\u03b2), \u03b1\u2010synuclein (\u03b1\u2010syn), and tau protein aggregates. We found that CLIP\u20101 exhibits markedly enhanced chemiluminescence (CL) in aqueous solution when activated by ambient oxygen in the presence of aggregated A\u03b2, whereas little to no emission is observed in the absence of aggregates or in the presence of monomeric A\u03b2. Additionally, CLIP\u20101 displayed a different wavelength of emission when bound to tau aggregates compared to A\u03b2 or \u03b1\u2010syn aggregates, which we attribute to differences in the relative overall charge of the amyloids at neutral pH. Imaging of brain slices confirmed enhanced CL of CLIP\u20101 in an Alzheimer\u2019s disease mouse model, highlighting the potential for amyloid\u2010targeting chemiluminescent probes as new tools for aiding in diagnosis of amyloid\u2010associated neurodegenerative diseases.<\/jats:p>","DOI":"10.1002\/cbic.202600008","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:35:26Z","timestamp":1777379726000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Charge\u2010Based Discrimination of Amyloids Using an Amyloid\u2010Targeting Chemiluminescent Probe"],"prefix":"10.1002","volume":"27","author":[{"given":"Jeewon","family":"Chung","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry University of California, San Diego  9500 Gilman Drive California La Jolla USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. Erin","family":"Jessup","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry University of California, San Diego  9500 Gilman Drive California La Jolla USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8423-7376","authenticated-orcid":false,"given":"Jerry","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry University of California, San Diego  9500 Gilman Drive California La Jolla USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"3708","DOI":"10.1002\/alz.13809","article-title":"2024 Alzheimer\u2019s Disease Facts and Figures","volume":"20","author":"Alzheimer\u2019s Association","year":"2024","journal-title":"Alzheimer\u2019s & Dementia"},{"issue":"7","key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"S10","DOI":"10.1038\/nm1066","article-title":"Protein Aggregation and Neurodegenerative Disease","volume":"10","author":"Ross C. 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Effect of pH on Rates of Spontaneous Autoxidation of CLA in Aqueous Buffer Solutions","volume":"66","author":"Fujimori K.","year":"1993","journal-title":"Bulletin of the Chemical Society of Japan"}],"container-title":["ChemBioChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.202600008","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbic.202600008","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.202600008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T05:59:33Z","timestamp":1777615173000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cbic.202600008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":30,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/cbic.202600008"],"URL":"https:\/\/doi.org\/10.1002\/cbic.202600008","archive":["Portico"],"relation":{},"ISSN":["1439-4227","1439-7633"],"issn-type":[{"value":"1439-4227","type":"print"},{"value":"1439-7633","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"2026-02-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202600008"}}