{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T10:41:00Z","timestamp":1777891260248,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["KO4687\/4\u20101"],"award-info":[{"award-number":["KO4687\/4\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:p>\n                    The determination of structural features is crucial to understand the interplay between structure and function of biomolecules and biomolecular complexes. In this context, nuclear magnetic resonance (NMR) spectroscopy provides experimental approaches, one of which is paramagnetic relaxation enhancement (PRE). Thus, placing a paramagnetic center and fluorine at strategic sites within (bio)molecules forming a complex enables the determination of distances through a straightforward, one\u2010dimensionally guided NMR spectroscopic setup. Moreover, the almost absence of fluorine in biomolecules found in nature allows performing experimental work using cell\u2010like or\n                    <jats:italic>in cell<\/jats:italic>\n                    conditions. Here, we made use of a single\u2010cysteine mutant of\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    cold shock protein B (\n                    <jats:italic>Bs<\/jats:italic>\n                    CspB) equipped with a paramagnetic spin label in complex with a fluorine\u2010labeled variant of singly stranded DNA ligand dT4 to acquire intermolecular,\n                    <jats:sup>19<\/jats:sup>\n                    F\u2010based PREs. The distance between\n                    <jats:italic>Bs<\/jats:italic>\n                    CspB and fluorine in dT4 has then been probed using three different experimental settings: in vitro, molecular crowding, and cell lysate conditions. Our data suggests that the intermolecular distance between the paramagnetically spin\u2010labeled protein and the fluorine\u2010labeled ligand does not change significantly using the three different conditions. This matches results regarding the conservation of binding affinities determined for this biomolecular complex using the three different conditions.\n                  <\/jats:p>","DOI":"10.1002\/cbic.70309","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T18:36:13Z","timestamp":1776450973000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Quantifying Ligand\u2010to\u2010Protein Distances in Complex Environments Using Intermolecular\n                    <sup>19<\/sup>\n                    F PRE NMR Spectroscopy"],"prefix":"10.1002","volume":"27","author":[{"given":"Yannick","family":"Werle","sequence":"first","affiliation":[{"name":"Department of Chemistry Universit\u00e4t Konstanz  Konstanz Germany"},{"name":"Graduate School of Chemical\u2010Biology (KoRS\u2010CB) Universit\u00e4t Konstanz  Konstanz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha\u2010Louise","family":"Inderfurth","sequence":"additional","affiliation":[{"name":"Department of Chemistry Universit\u00e4t Konstanz  Konstanz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher J.","family":"Lang","sequence":"additional","affiliation":[{"name":"Department of Chemistry Universit\u00e4t Konstanz  Konstanz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3357-9843","authenticated-orcid":false,"given":"Michael","family":"Kovermann","sequence":"additional","affiliation":[{"name":"Department of Chemistry Universit\u00e4t Konstanz  Konstanz Germany"},{"name":"Graduate School of Chemical\u2010Biology (KoRS\u2010CB) Universit\u00e4t Konstanz  Konstanz Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008320"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.2661"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006266"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2022.04.005"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbinf.2022.983306"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-023-02087-4"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3002187"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnmrs.2020.12.001"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mie.2015.06.032"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(92)90529-S"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.2207"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7386-6_12"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00249-017-1257-z"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.106.090308"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr900033p"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-458X(200007)38:7<551::AID-MRC686>3.0.CO;2-Q"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.202402820"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-66265-w"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202000413"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.3c00545"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201508464"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201900453"},{"key":"e_1_2_8_25_1","first-page":"2160","article-title":"19F Paramagnetic Relaxation\u2010Based NMR for Quaternary Structural Restraints of Ion Channels","volume":"14","author":"Bondarenko V.","year":"2019","journal-title":"Acs Chemical Biology"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202201097"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-97219-1"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202100304"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmr.2015.08.009"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1259\/bjr\/17326033"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl376"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201800679"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmr.2018.12.005"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(99)77427-8"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1994.0073"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00012a001"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201301657"}],"container-title":["ChemBioChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.70309","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbic.70309","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.70309","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T05:58:42Z","timestamp":1777615122000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cbic.70309"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":36,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/cbic.70309"],"URL":"https:\/\/doi.org\/10.1002\/cbic.70309","archive":["Portico"],"relation":{},"ISSN":["1439-4227","1439-7633"],"issn-type":[{"value":"1439-4227","type":"print"},{"value":"1439-7633","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"2026-02-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70309"}}