{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T10:40:51Z","timestamp":1777891251223,"version":"3.51.4"},"reference-count":66,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:p>\n                    We report a series of uracil\u2010triazole\u2013pyrene peptidomimetics (\n                    <jats:bold>2<\/jats:bold>\n                    \u2013\n                    <jats:bold>4<\/jats:bold>\n                    ) designed to achieve modular geometric control through peptide linkers of varying length and flexibility. All three compounds exhibited submicromolar affinity for ds\u2010DNA, while compound\n                    <jats:bold>4<\/jats:bold>\n                    also showed strong binding to ds\u2010RNA, demonstrating the advantage of combining a uracil recognition unit with an extended pyrene aromatic surface compared to the\n                    <jats:bold>Pyr\u2010Trp<\/jats:bold>\n                    reference ligand and the phenanthridine analog\n                    <jats:bold>4\u2032<\/jats:bold>\n                    . Structural variations strongly affected Cu(II) coordination. Although\n                    <jats:bold>Pyr\u2010Trp<\/jats:bold>\n                    bound Cu(II) approximately 1000\u2010fold more strongly than the short, rigid compound\n                    <jats:bold>2<\/jats:bold>\n                    , elongation and increased linker flexibility restored high affinity in\n                    <jats:bold>4<\/jats:bold>\n                    . Notably, replacing phenanthridine (\n                    <jats:bold>4\u2032<\/jats:bold>\n                    ) with pyrene (\n                    <jats:bold>4<\/jats:bold>\n                    ) enhanced Cu(II) binding by nearly 100\u2010fold, highlighting the superior coordination properties of the pyrene scaffold. Cu(II) complexation significantly enhanced nucleic acid binding exclusively for compound\n                    <jats:bold>2<\/jats:bold>\n                    , increasing its DNA\/RNA affinity 15\u2010fold. The resulting\n                    <jats:bold>2<\/jats:bold>\n                    \u2013Cu(II) complex displayed exceptional selectivity for poly rA\u2013poly rU, exceeding that of the\n                    <jats:bold>Pyr\u2010Trp<\/jats:bold>\n                    \u2013Cu(II) analog by more than 10\u2010fold, consistent with uracil\u2010mediated Hoogsteen\u2010type recognition. Membrane interactions with POPC MLVs were linker\u2010dependent, producing fluorescence enhancements of up to 1400% (\n                    <jats:bold>2<\/jats:bold>\n                    ), 650% (\n                    <jats:bold>3<\/jats:bold>\n                    ), and 100% (\n                    <jats:bold>4<\/jats:bold>\n                    ). Together with negligible cytotoxicity, these findings indicate that compounds\n                    <jats:bold>2<\/jats:bold>\n                    \u2013\n                    <jats:bold>4<\/jats:bold>\n                    represent promising multifunctional platforms for Cu\n                    <jats:sup>2+<\/jats:sup>\n                    sensing and photoinduced therapeutic applications.\n                  <\/jats:p>","DOI":"10.1002\/cbic.70312","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T18:41:41Z","timestamp":1776451301000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Uracil\u2013Peptide\u2013Pyrene Conjugates as Cu\n                    <sup>2+<\/sup>\n                    \u2010Sensitive Probes Targeting DNA, RNA, and Biorelevant Model Membranes"],"prefix":"10.1002","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0657-1635","authenticated-orcid":false,"given":"Dijana","family":"Pavlovi\u0107 Safti\u0107","sequence":"first","affiliation":[{"name":"Division of Organic Chemistry and Biochemistry Ru\u0111er Bo\u0161kovi\u0107 Institute  Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucija","family":"Luli\u0107 Horvat","sequence":"additional","affiliation":[{"name":"Division of Molecular Biology Ru\u0111er Bo\u0161kovi\u0107 Institute  Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivo","family":"Piantanida","sequence":"additional","affiliation":[{"name":"Division of Organic Chemistry and Biochemistry Ru\u0111er Bo\u0161kovi\u0107 Institute  Zagreb 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