{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:47:19Z","timestamp":1790912839814,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:p>\n                    Antimicrobial resistance (AMR) has emerged as a global healthcare crisis, necessitating the discovery of potent antibacterial agents. In the present investigation, we report the discovery, in\u2009vitro and ex vivo antibacterial efficacy studies, and mechanism of action of an unexplored benzimidazole derivative (\n                    <jats:bold>BI\u201010<\/jats:bold>\n                    ), a promising broad\u2010spectrum antibacterial agent with significant efficacy against\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    and methicillin\u2010resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (MRSA).\n                    <jats:bold>BI\u201010<\/jats:bold>\n                    demonstrated a minimum Inhibitory Concentration (MIC) of 2.4\u2009\u03bcg\/ml (6.25\u2009\u00b5M) against these priority pathogens. In vitro assessments revealed that\n                    <jats:bold>BI\u201010<\/jats:bold>\n                    possessed rapid bactericidal activity, anti\u2010biofilm potential and showed synergistic interactions with conventional antibiotics. Ex\u2009vivo efficacy studies using various mammalian cell lines demonstrated strong intracellular killing. Moreover,\n                    <jats:bold>BI\u201010<\/jats:bold>\n                    showed the ability to prevent both adhesion and invasion of pathogens in different mammalian cell infection models. The membrane\u2010disrupting nature of\n                    <jats:bold>BI\u201010<\/jats:bold>\n                    against both pathogens was established using scanning electron microscopy, membrane permeability, depolarization, and integrity assays. Multiple in\u2009silico analyses further demonstrated drug\u2010likeness and the biocompatibility profile of\n                    <jats:bold>BI\u201010<\/jats:bold>\n                    . Altogether, our findings establish\n                    <jats:bold>BI\u201010<\/jats:bold>\n                    as a potent, broad\u2010spectrum antimicrobial agent with robust in vitro and ex\u2009vivo efficacy and bacterial cell membrane\u2010disrupting activity. This study highlights\n                    <jats:bold>BI\u201010<\/jats:bold>\n                    as a valuable antibacterial lead molecule for the development of a new antimicrobial agent against multidrug\u2010resistant pathogens.\n                  <\/jats:p>","DOI":"10.1002\/cbic.70316","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T06:54:45Z","timestamp":1776754485000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Discovery of BI\u201010: An Unexplored Broad\u2010Spectrum Antimicrobial Benzimidazole Derivative Targeting Bacterial Membrane"],"prefix":"10.1002","volume":"27","author":[{"given":"Biplab","family":"Sarkar","sequence":"first","affiliation":[{"name":"Infectious Diseases Division CSIR\u2010Indian Institute of Integrative Medicine  Jammu Jammu and Kashmir India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rakshit","family":"Manhas","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division CSIR\u2010Indian Institute of Integrative Medicine  Jammu Jammu and Kashmir India"},{"name":"Academy of Scientific &amp; Innovative Research (AcSIR)  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arti","family":"Rathore","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division CSIR\u2010Indian Institute of Integrative Medicine  Jammu Jammu and Kashmir India"},{"name":"Academy of Scientific &amp; Innovative Research (AcSIR)  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyoti","family":"Kumari","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division CSIR\u2010Indian Institute of Integrative Medicine  Jammu Jammu and Kashmir India"},{"name":"Academy of Scientific &amp; Innovative Research (AcSIR)  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shifa","family":"Firdous","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division CSIR\u2010Indian Institute of Integrative Medicine  Jammu Jammu and Kashmir India"},{"name":"Academy of Scientific &amp; Innovative Research (AcSIR)  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5140-5986","authenticated-orcid":false,"given":"Avisek","family":"Mahapa","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division CSIR\u2010Indian Institute of Integrative Medicine  Jammu Jammu and Kashmir India"},{"name":"Academy of Scientific &amp; Innovative Research (AcSIR)  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,20]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2024.105073"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1086\/590937"},{"key":"e_1_2_10_4_1","first-page":"ofz503","volume-title":"IESKAPE Pathogens in Bloodstream Infections Are Associated with Higher Cost and Mortality but Can Be Predicted Using Diagnoses upon Admission","author":"Marturano J. 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