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The biocatalytic route couples\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010glucosidase for deglycosylation with laccase and xanthine oxidase (XO) for subsequent oxidation. The primary objective of this approach was to assess the feasibility of enzyme coupling within a single one\u2010pot reaction. The results demonstrate that this strategy successfully enabled the transformation of salicylic alcohol into salicylic acid, mediated by a compatible laccase\/TEMPO and XO system in a one\u2010step process at pH 7 and 25\u00b0C. However, the integration of\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010glucosidase into a one\u2010pot system for direct salicin conversion was found to reduce the oxidative efficiency of XO, thus necessitating a sequential approach. Applying this optimized enzymatic cascade to a crude\n                    <jats:italic>Salix purpurea<\/jats:italic>\n                    bark extract initially encountered limitations in oxidation due to matrix complexity. Subsequent optimization of biocatalyst dosage delivered complete substrate oxidation and a 95% salicylic acid yield. This research significantly broadens the applicability of multienzymatic biocatalytic cascades, offering a promising and sustainable route for transforming natural compounds within complex plant extracts into high\u2010value bioactive ingredients.\n                  <\/jats:p>","DOI":"10.1002\/cbic.70348","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T11:50:08Z","timestamp":1776772208000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Novel Multienzymatic Cascade for the Conversion of Salicin From Plant Extract Into Salicylic Acid"],"prefix":"10.1002","volume":"27","author":[{"given":"Amandine","family":"Moreno","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Reims Champagne\u2010Ardenne INRAE, FARE, UMR A 614, AFERE  Reims France"},{"name":"Nov\u00e9al \u2013 groupe L\u2019Or\u00e9al  Le Thillay France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louis\u2010Nicolas","family":"Gombault","sequence":"additional","affiliation":[{"name":"Nov\u00e9al \u2013 groupe L\u2019Or\u00e9al  Le Thillay France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrien","family":"Cottias","sequence":"additional","affiliation":[{"name":"Nov\u00e9al \u2013 groupe L\u2019Or\u00e9al  Le Thillay France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"R\u00e9mond","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Reims Champagne\u2010Ardenne INRAE, FARE, UMR A 614, AFERE  Reims France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23071571"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.btre.2019.e00370"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13820"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erae309"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.878076"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s44372-025-00274-5"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apcata.2008.03.037"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.5620\/eaht.2025007"},{"key":"e_1_2_10_10_1","unstructured":"Y.YanandY.Lin US 9719113 (2007)."},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2025.120651"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcat.2022.112187"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jscs.2010.04.010"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2021.113976"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/782763"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2024.05.009"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2021.103859"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.889184"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/np50087a006"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cattod.2023.114277"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cctc.201403040"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/catal7120354"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2022.133114"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsos.211572"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202309012"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-016-7987-5"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2005.12.076"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201800343"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/catal10090952"},{"key":"e_1_2_10_30_1","first-page":"3","article-title":"Immobilized Enzymatic Alcohol Oxidation as a Versatile Reaction Module for Multienzyme Cascades","volume":"4","author":"Fu K.","year":"2024","journal-title":"Chemical Synthesis"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4GC01321B"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/cssc.201402136"},{"key":"e_1_2_10_33_1","doi-asserted-by":"crossref","unstructured":"W. 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