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With the goal of making UPO an industrial biocatalyst, it has been subjected over the years to different directed evolution campaigns focused on expression, stability, activity, and selectivity. In this work, we tested the ability of PaDa\u2010I and JaWa, evolved variants of the UPO from\n                    <jats:italic>Agrocybe aegerita<\/jats:italic>\n                    , to catalyze the oxidation of a series of pharmaceutical compounds, considered emerging contaminants (EC), or microcontaminants. Remarkably, these UPO mutants were able to catalyze the oxidation of 9 out of 12 EC, albeit with different efficacy. A detailed analysis of the reaction efficiency and the nontoxic nature of the reaction products point these UPO mutants as promising tools for enzymatic bioremediation schemes.\n                  <\/jats:p>","DOI":"10.1002\/cbic.70351","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T14:38:54Z","timestamp":1777041534000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unspecific Peroxygenases\u2010Catalyzed Oxidation of Pharmaceutical Compounds Considered Emerging Contaminants"],"prefix":"10.1002","volume":"27","author":[{"given":"Alina","family":"Torres\u2010Aguirre","sequence":"first","affiliation":[{"name":"Departamento de Ingenier\u00eda Celular y Biocat\u00e1lisis Instituto de Biotecnolog\u00eda, UNAM  Cuernavaca Morelos M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Azucena","family":"L\u00f3pez\u2010L\u00f3pez","sequence":"additional","affiliation":[{"name":"Departamento de Ingenier\u00eda Celular y Biocat\u00e1lisis Instituto de Biotecnolog\u00eda, UNAM  Cuernavaca Morelos M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayra","family":"Avelar","sequence":"additional","affiliation":[{"name":"Laboratorio de S\u00edntesis Asim\u00e9trica y Bio\u2010Quimioinform\u00e1tica (LSAyB) Ingenier\u00eda Qu\u00edmica (UACQ) Universidad Aut\u00f3noma de Zacatecas  Zacatecas M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6780-7616","authenticated-orcid":false,"given":"Miguel","family":"Alcalde","sequence":"additional","affiliation":[{"name":"Departamento de Biocat\u00e1lisis Instituto de Cat\u00e1lisis CSIC  Madrid Espa\u00f1a"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1234-4287","authenticated-orcid":false,"given":"Marcela","family":"Ayala","sequence":"additional","affiliation":[{"name":"Departamento de Ingenier\u00eda Celular y Biocat\u00e1lisis Instituto de Biotecnolog\u00eda, UNAM  Cuernavaca Morelos M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gsd.2017.12.009"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2005.02.016"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2005.10.018"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etap.2009.01.002"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2004.11.090"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etap.2013.09.009"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/etc.3339"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.3.646-666.1998"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jclepro.2019.02.135"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.2166\/9781789063752_0203"},{"key":"e_1_2_9_12_1","unstructured":"N.Morin\u2010Crini E.Lichtfouse M.Fourmentin et al. 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