{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T10:40:53Z","timestamp":1777891253455,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"KAKENHI","doi-asserted-by":"publisher","award":["15H01810"],"award-info":[{"award-number":["15H01810"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:p>Fluorogenic probes are valuable tools for rapid detection and bioimaging of target molecules without requiring bound\/free separation. Although antibody\u2010based probes are widely used, their large size, reliance on secondary labeling, and limited accessibility to some intracellular regions can restrict their utility. In contrast, fluorogenic peptide aptamers\u2014compact, chemically synthesizable, and self\u2010reporting\u2014offer a promising alternative. Here, we developed a method to prepare a fluorophore\u2010conjugated peptide library using cDNA display and applied it to the selection of fluorogenic peptides targeting heat shock protein 90\u03b1 (Hsp90\u03b1), a clinically relevant biomarker. As the solvatochromic reporter, we used an environmentally sensitive fluorophore, 4\u2010N, N\u2010dimethylamino\u20101,8\u2010naphthalimide (4\u2010DMN). One selected peptide, Peptide2, exhibited fluorescence enhancement in the presence of Hsp90\u03b1 while remaining largely unresponsive to the homologous Hsp70. In fixed cells, Peptide2 produced intracellular fluorescence patterns that partially overlapped with those obtained using an anti\u2010Hsp90\u03b1 antibody, supporting its ability to detect intracellular Hsp90\u03b1\u2010related signals. Peptide2 and the antibody also showed partially distinct staining distributions, suggesting differences in epitope accessibility and\/or intracellular accessibility. These results establish a proof of concept for generating target\u2010responsive, self\u2010reporting peptide probes using bioorthogonal cDNA display.<\/jats:p>","DOI":"10.1002\/cbic.70360","type":"journal-article","created":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T05:53:51Z","timestamp":1777096431000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fluorogenic Peptide Aptamer Generated Using Bioorthogonal cDNA Display Technology for Cellular Imaging of Heat Shock Protein 90\u03b1"],"prefix":"10.1002","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6042-513X","authenticated-orcid":false,"given":"Takanori","family":"Uzawa","sequence":"first","affiliation":[{"name":"Emergent Bioengineering Materials Research Team RIKEN Center for Emergent Matter Science  Wako Saitama Japan"},{"name":"Nano Medical Engineering Laboratory RIKEN Cluster for Pioneering Research  Wako Saitama 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Engineering Laboratory RIKEN Cluster for Pioneering Research  Wako Saitama Japan"},{"name":"School of Basic Medical Sciences Lanzhou University  Lanzhou Gansu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9231-8128","authenticated-orcid":false,"given":"Gang","family":"Huang","sequence":"additional","affiliation":[{"name":"Nano Medical Engineering Laboratory RIKEN Cluster for Pioneering Research  Wako Saitama Japan"},{"name":"Human Phenome Institute Zhangjiang Fudan International Innovation Center Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Nano Medical Engineering Laboratory RIKEN Cluster for Pioneering Research  Wako Saitama Japan"},{"name":"Institute of Health and Medical Technology Hefei Institutes of Physical Science Chinese Academy of Sciences  Hefei PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2038-5145","authenticated-orcid":false,"given":"Naoto","family":"Nemoto","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Engineering Saitama University  Sakura\u2010ku Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1154-253X","authenticated-orcid":false,"given":"Yoshihiro","family":"Ito","sequence":"additional","affiliation":[{"name":"Emergent Bioengineering Materials Research Team RIKEN Center for Emergent Matter Science  Wako Saitama Japan"},{"name":"Nano Medical Engineering Laboratory RIKEN Cluster for Pioneering Research  Wako Saitama 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