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Transition\u2010metal complexes have emerged as attractive possibilities owing to their varied geometries, redox activity, and capacity to interact with biological sites inaccessible to traditional chemical antibiotics. This study presents the antibacterial and antifungal assessment of a series of Ru(II) pyrazolyl\u2013pyridine half\u2010sandwich complexes (\n                    <jats:bold>C1<\/jats:bold>\n                    \u2013\n                    <jats:bold>C6<\/jats:bold>\n                    ), thereby broadening the chemical scope of metalloantibiotics previously investigated inside pyridyl\u20131,2,3\u2010triazolyl frameworks. The compounds were synthesized with good yields and structurally validated by single\u2010crystal X\u2010ray diffraction, demonstrating piano\u2010stool topologies with unique ligand\u2010dependent anion orientations. Extensive biological screening via the Community for Open Antimicrobial Drug Discovery (CO\u2010ADD) platform revealed selective efficacy of complexes\n                    <jats:bold>C1<\/jats:bold>\n                    \u2013\n                    <jats:bold>C3<\/jats:bold>\n                    against Acinetobacter baumannii, a significant multidrug\u2010resistant pathogen. Cytotoxicity and hemolysis assessments revealed advantageous therapeutic ranges for the most potent combinations. Biophysical investigations, encompassing DNA\u2010binding fluorescence displacement, linear dichroism, and in silico molecular docking and dynamics, elucidated that the lead drug interacts with bacterial DNA via partial intercalation and groove contacts. Collectively, our findings establish Ru(II) pyrazolyl\u2013pyridine complexes as prospective candidates for the advancement of next\u2010generation metalloantibiotics and underscore the significance of coordination\u2010chemistry\u2010driven approaches in addressing AMR.\n                  <\/jats:p>","DOI":"10.1002\/cbic.70371","type":"journal-article","created":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T18:39:42Z","timestamp":1778438382000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Pyrazolyl\u2013Pyridine Ruthenium Complexes: A New Metallic Line of Defense Against\n                    <i>Acinetobacter baumannii<\/i>"],"prefix":"10.1002","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1732-5592","authenticated-orcid":false,"given":"M. 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