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While\n                    <jats:bold>2\u2010NBDG<\/jats:bold>\n                    , a fluorescent glucose analog, enables GLUT\u2010mediated imaging, it suffers from low brightness and requires high concentrations and washing. Herein, we report the development of turn\u2010on fluorescent probes for GLUTs based on the viscosity\u2010sensitive fluorophore in GFP for a wash\u2010free imaging of prostate cancer cell, PC\u20103. A series of fluorescent molecular rotors (FMRs) were synthesized by modifying the 2\u2010methyl\u20104\u2010(\n                    <jats:italic>p<\/jats:italic>\n                    \u2010dimethylaminobenzylidene)\u20105\u2010imidazolinone (\n                    <jats:bold>DMAB<\/jats:bold>\n                    ) core. Structural modifications, such as cyclic amine substitution and julolidine incorporation, enhanced fluorescence quantum yields (\u03a6\n                    <jats:sub>F<\/jats:sub>\n                    up to 0.17 in glycerol) and viscosity sensitivity (\n                    <jats:italic>\u03c7<\/jats:italic>\n                    up to 0.87). Among them, the julolidine\u2010containing fluorophore\n                    <jats:bold>Julo\u2010Ph<\/jats:bold>\n                    showed the best performance. The\n                    <jats:bold>DMAB<\/jats:bold>\n                    \u2010based fluorophores were conjugated to glucose or glucosamine to create GLUT\u2010targeted probes. A glucosamine\u2010bound conjugate,\n                    <jats:bold>GlcN\u2010Julo\u2010Ph,<\/jats:bold>\n                    was found to enable a wash\u2010free bright fluorescence imaging of PC\u20103 cancer cells. Inhibition studies and docking simulations strongly suggested a GLUT\u2010mediated uptake of the fluorophore.\n                    <jats:bold>GlcN\u2010Julo\u2010Ph<\/jats:bold>\n                    outperformed\n                    <jats:bold>2\u2010NBDG<\/jats:bold>\n                    in emitting a brighter intracellular fluorescence at a lower concentration, notably, under wash\u2010free conditions. Our findings shed light on the utility of viscosity\u2010sensitive FMRs for the design of turn\u2010on imaging probes and offer a promising platform for a rapid, low\u2010background cancer cell detection.\n                  <\/jats:p>","DOI":"10.1002\/cbic.70372","type":"journal-article","created":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T05:54:14Z","timestamp":1779083654000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Glucose\u2010Modified Viscosity\u2010Responsive Turn\u2010On Fluorescence Molecular Rotors for Wash\u2010Free Live\u2010Cell Imaging"],"prefix":"10.1002","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1782-9283","authenticated-orcid":false,"given":"Takashi","family":"Kanamori","sequence":"first","affiliation":[{"name":"School of Life Science and Technology Institute of Science Tokyo  Yokohama 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Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroya","family":"Asami","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Science Gakushuin University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun\u2010ichiro","family":"Ogura","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology Institute of Science Tokyo  Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideya","family":"Yuasa","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology Institute of Science Tokyo  Yokohama 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