{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T11:07:50Z","timestamp":1779880070299,"version":"3.53.1"},"reference-count":52,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001804","name":"Canada Research Chairs","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001804","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2026,5,27]]},"abstract":"<jats:p>Folate (vitamin B9) is an essential micronutrient for human health, particularly during pregnancy. Aptamers have emerged as attractive recognition elements for small\u2010molecule detection, as demonstrated by numerous natural riboswitches. Although an RNA aptamer for folate has been reported, the development of a DNA aptamer is highly desirable for biosensing applications due to its superior chemical stability and ease of modification. Herein, we employed a library\u2010immobilization\u2010based selection strategy to isolate a family of high\u2010affinity DNA aptamers for folate. Isothermal titration calorimetry (ITC) measurements yielded a dissociation constant of 640\u2009nM. Based on the selected aptamer (FA1), we developed a signal\u2010on, strand\u2010displacement fluorescent biosensor by hybridizing a 5\u2032\u2010FAM\u2010labeled aptamer with a short quencher\u2010labeled complementary strand. The sensor achieved a limit of detection (LOD) of 8.5\u2009nM in selection buffer and 31\u2009nM in 10% serum. Selectivity studies demonstrated negligible responses to structurally related compounds, including guanine, adenine, theophylline, xanthine, and caffeine. Tetrahydrofolate generated a measurable signal only at elevated concentrations; however, ITC confirmed no direct binding, indicating that the observed response likely arises from its oxidation to folate. These results establish a robust DNA aptamer for folate for both fundamental studies and applied contexts, such as clinical monitoring and food fortification quality control.<\/jats:p>","DOI":"10.1002\/cbic.70393","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T07:42:07Z","timestamp":1779694927000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Capture\u2010SELEX of DNA Aptamers for Highly Selective Binding of Folate"],"prefix":"10.1002","volume":"27","author":[{"given":"Yachen","family":"Xie","sequence":"first","affiliation":[{"name":"Department of Chemistry Waterloo Institute for Nanotechnology University of Waterloo  Waterloo Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Chemistry Waterloo Institute for Nanotechnology University of Waterloo  Waterloo Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5918-9336","authenticated-orcid":false,"given":"Juewen","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Chemistry Waterloo Institute for Nanotechnology University of Waterloo  Waterloo Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"2284","DOI":"10.1021\/ja01853a512","article-title":"The Concentration of \u201cFolate\u201d","volume":"63","author":"Herschel K.","year":"1941","journal-title":"Journal of the American Chemical 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