{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T12:24:59Z","timestamp":1788265499372,"version":"build-2803163510"},"reference-count":44,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2026,7,29]]},"abstract":"<jats:p>\n                    Efficient, selective, and sustainable synthetic platforms are increasingly important in modern chemical manufacturing, yet chemical flexibility often remains limited. In this study, we develop a modular biocatalytic system for the divergent and continuous transformation of ethyl acetoacetate (EAA) into either enantiomerically pure (\n                    <jats:italic>R<\/jats:italic>\n                    )\u20103\u2010hydroxybutyric acid ((\n                    <jats:italic>R<\/jats:italic>\n                    )\u20103HBA) or oligomers of (\n                    <jats:italic>R<\/jats:italic>\n                    )\u2010poly(3\u2010hydroxybutyrate) (PHB). The cascade combines two telescoped packed\u2010bed reactors containing an immobilized, self\u2010sufficient\n                    <jats:italic>Lactobacillus kefir<\/jats:italic>\n                    ketoreductase (\n                    <jats:italic>Lk<\/jats:italic>\n                    KRED) and commercial immobilized\n                    <jats:italic>Candida antarctica<\/jats:italic>\n                    lipase B (N435). By simply tuning the reaction conditions, the system selectively directs the substrate toward either product. Under aqueous conditions, it achieves quantitative conversion to (\n                    <jats:italic>R<\/jats:italic>\n                    )\u20103HBA with &gt;99% enantiomeric excess and maintains excellent stability over 7 days of continuous operation. It also reaches a maximum space\u2013time yield of 105\u2009g\u2009L\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u2009h\n                    <jats:sup>\u22121<\/jats:sup>\n                    , which is 33 times higher than the volumetric productivity of the sequential two\u2010pot process. Under anhydrous conditions, the same setup promotes polycondensation into PHB oligomers, as confirmed by matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight analysis. Overall, this platform combines productivity, stereoselectivity, and flexibility for the integrated synthesis of chiral building blocks or oligomers.\n                  <\/jats:p>","DOI":"10.1002\/cbic.70474","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T12:24:19Z","timestamp":1784895859000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Continuous and Divergent Biotransformation of \u03b2\u2010Ketoesters Into Enantiomerically Pure \u03b2\u2010Hydroxyacids or Their Oligomers Through a Telescoped Multienzyme Cascade"],"prefix":"10.1002","volume":"27","author":[{"given":"Giulio","family":"Pota","sequence":"first","affiliation":[{"name":"Heterogeneous Biocatalysis Laboratory Center for Cooperative Research in Biomaterials (CIC biomaGUNE) Basque Research and Technology Alliance (BRTA)  Donostia\/San Sebasti\u00e1n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Andr\u00e9s\u2010Sanz","sequence":"additional","affiliation":[{"name":"Heterogeneous Biocatalysis Laboratory Center for Cooperative Research in Biomaterials (CIC biomaGUNE) Basque Research and Technology Alliance (BRTA)  Donostia\/San Sebasti\u00e1n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ainhoa","family":"Maiz\u2010Iginitz","sequence":"additional","affiliation":[{"name":"Heterogeneous Biocatalysis Laboratory Center for Cooperative Research in Biomaterials (CIC biomaGUNE) Basque Research and Technology Alliance (BRTA)  Donostia\/San Sebasti\u00e1n Spain"},{"name":"POLYMAT Department of Polymer Science and Technology University of the Basque Country UPV\/EHU  Donostia\/San Sebastian Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana A.","family":"Armend\u00e1riz\u2010Ruiz","sequence":"additional","affiliation":[{"name":"Heterogeneous Biocatalysis Laboratory Center for Cooperative Research in Biomaterials (CIC biomaGUNE) Basque Research and Technology Alliance (BRTA)  Donostia\/San Sebasti\u00e1n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haritz","family":"Sardon","sequence":"additional","affiliation":[{"name":"POLYMAT Department of Polymer Science and Technology University of the Basque Country UPV\/EHU  Donostia\/San Sebastian Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Vitiello","sequence":"additional","affiliation":[{"name":"Department of Chemical, Materials and Production Engineering University of Naples Federico II  Naples Italy"},{"name":"Center for Colloid and Surface Science (CSGI)  Sesto Fiorentino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Califano","sequence":"additional","affiliation":[{"name":"Institute of Science and Technology for Sustainable Energy and Mobility (STEMS) National Research Council of Italy (CNR)  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aniello","family":"Costantini","sequence":"additional","affiliation":[{"name":"Department of Chemical, Materials and Production Engineering University of Naples Federico II  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"L\u00f3pez\u2010Gallego","sequence":"additional","affiliation":[{"name":"Heterogeneous Biocatalysis Laboratory Center for Cooperative Research in Biomaterials (CIC biomaGUNE) Basque Research and Technology Alliance (BRTA)  Donostia\/San Sebasti\u00e1n Spain"},{"name":"IKERBASQUE, Basque Foundation for Science  Bilbao 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