{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T16:35:58Z","timestamp":1786725358927,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000001","name":"U.S. National Science Foundation","doi-asserted-by":"publisher","award":["CHE\u20101903899"],"award-info":[{"award-number":["CHE\u20101903899"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2026,8,14]]},"abstract":"<jats:p>\n                    Pyridoxal\u20105\u2032\u2010phosphate (PLP)\u2010dependent L\u2010arginine oxidases (PDAOs) catalyze 4\u2010electron oxidations of L\u2010arginine in the context of secondary metabolite biosynthesis. PDAOs are separated into two classes based on whether they hydroxylate or desaturate L\u2010arginine. A detailed mechanistic understanding of these enzymes will extend our knowledge of natural products biosynthesis. Here, we present detailed transient kinetic analyses of representative hydroxylating and desaturating PDAOs: MppP from\n                    <jats:italic>Streptomyces wadayamensis<\/jats:italic>\n                    (SwMppP) and the Ind4 homolog from\n                    <jats:italic>Pseudoalteromonas luteoviolacea<\/jats:italic>\n                    (Plu4). While both SwMppP and Plu4 kinetics can be accurately predicted using the same kinetic model, they in fact use subtly different mechanisms. This work seeks to uncover how two enzymes that are so similar in terms of sequence, structure, and catalytic function can have different mechanisms. To this end, we determined the structure of an additional desaturating PDAO, the Ind4 homolog from\n                    <jats:italic>Bacillus cereus<\/jats:italic>\n                    (Bcer4). This brings the total number of PDAOs whose structures have been determined to four: two hydroxylases and two desaturases. Examination of the available structural data fails to point out any class\u2010specific structural differences that could explain the mechanistic differences between the classes. The subtle mechanistic differences are likely due to differences in protein dynamics rather than structure.\n                  <\/jats:p>","DOI":"10.1002\/cbic.70488","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T19:24:46Z","timestamp":1785957886000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Toward an Understanding of the Mechanistic Differences Between the Hydroxylating and Desaturating Pyridoxal 5\u2032\u2010Phosphate\u2010Dependent L\u2010Arginine Oxidases"],"prefix":"10.1002","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7566-1346","authenticated-orcid":false,"given":"Trevor R.","family":"Melkonian","sequence":"first","affiliation":[{"name":"Division of Chemical Biology and Medicinal Chemistry College of Pharmacy The University of Texas at Austin  Austin Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2883-6312","authenticated-orcid":false,"given":"Nemanja","family":"Vuksanovic","sequence":"additional","affiliation":[{"name":"Department of Chemistry Boston University  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8050-1882","authenticated-orcid":false,"given":"Lanlan","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Bioinformatics School of Science Xi\u2019an Jiaotong\u2010Liverpool University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3941-0376","authenticated-orcid":false,"given":"Zachary A.","family":"Uttke","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Cell Biology, and Biochemistry Warren Alpert Medical School Brown University  Providence Rhode Island USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0576-0714","authenticated-orcid":false,"given":"Nicholas R.","family":"Silvaggi","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry University of Wisconsin\u2013Milwaukee  Milwaukee Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.5b01016"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2009"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00246a002"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi800478s"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470920541.ch7"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M602708200"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.8b00130"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.8b00039"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2012591118"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.15277"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2025.110605"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.17.4591-4595.1998"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1983.tb07087.x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.6c00271"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mie.2023.03.010"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mie.2019.03.005"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(09)67023-3"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2008.12.025"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2008.12.024"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1107\/S2059798324001487"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444911007773"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909047337"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1107\/S090744491003982X"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444913000061"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1107\/S2059798323003595"},{"key":"e_1_2_9_27_1","unstructured":"P.Zwart R.Grosse\u2010Kunsteleve andP.Adams CCP4 Newsletter (2005)."},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03819-2"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889807021206"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.4992"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1107\/S205979832300102X"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909052925"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1107\/S2059798319011471"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444912001308"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910007493"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10969-005-3138-4"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm216"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.2400"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btae370"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki524"}],"container-title":["ChemBioChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.70488","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbic.70488","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.70488","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T16:02:18Z","timestamp":1786723338000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cbic.70488"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,5]]},"references-count":39,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2026,8,14]]}},"alternative-id":["10.1002\/cbic.70488"],"URL":"https:\/\/doi.org\/10.1002\/cbic.70488","archive":["Portico"],"relation":{},"ISSN":["1439-4227","1439-7633"],"issn-type":[{"value":"1439-4227","type":"print"},{"value":"1439-7633","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,5]]},"assertion":[{"value":"2026-03-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70488"}}