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Our previous studies have demonstrated that incorporating basic fibroblast growth factor (bFGF) and urine\u2010derived stem cells (USCs) into bladder acellular matrix (BAM) enhances bladder repair. While these approaches have shown promising results in regenerating bladder epithelium and smooth muscle, the outcomes of neural regeneration are still not satisfactory. Therefore, this study introduces the 1\u201314aa sequence from the NGF polypeptide (NMP), a small molecule NGF peptidomimetic, which could improve neural regeneration in the body. Utilizing the tissue inhibitors of metalloproteinases (TIMP) that is cleaved by MMP\u20102 to design an MMP\u20102 responsive NMP delivery system, which could control the release of NMP according to the amount of MMP\u20102 in the local microenvironment. By chemical cross\u2010linking, NMP\u2010TIMP and bFGF were conjugated with BAM seeded with USCs to compose BAM\/USCs\/bFGF\/NMP\u2010TIMP functional biomaterial. Using a rat bladder reconstruction model, the functional biomaterial significantly enhanced the regeneration of endothelial cells, capillaries, smooth muscle cells, nerve fibers, and the bladder functions. In summary, the BAM\/USCs\/bFGF\/NMP\u2010TIMP functional biomaterial may offer a promising strategy for tissue\u2010engineered bladder by promoting bladder regeneration, especially the regeneration of bladder nerves.<\/jats:p>","DOI":"10.1002\/cbic.70500","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T10:09:09Z","timestamp":1788862149000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["MMP\u20102 Responsive Functional Bladder Acellular Matrix Scaffold Combined With Urine Derived Stem Cells for Bladder Nerve Regeneration"],"prefix":"10.1002","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1239-6318","authenticated-orcid":false,"given":"Hao","family":"Xia","sequence":"first","affiliation":[{"name":"Department of Urology Jiangbei Campus of The First Affiliated Hospital of Army Medical University (The 958th Hospital of Chinese People's Liberation Army)  Chongqing 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