{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T05:17:59Z","timestamp":1787894279548,"version":"build-2784847793"},"reference-count":40,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22278224"],"award-info":[{"award-number":["22278224"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22478191"],"award-info":[{"award-number":["22478191"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2026,8,27]]},"abstract":"<jats:p>\n                    Stimuli\u2010responsive prodrug system has emerged as an attractive approach to address the issues encountered by conventional chemotherapeutics. Among the growing arsenal of stimuli\u2010responsive prodrug strategies, the photocaging approach occupies a privileged position because it replaces poorly controlled biological triggers with an external light stimulation, thereby offering a promising solution to the therapeutic limitations in cancer treatment. In this work, we have developed a Sanger\u2019s reagent based prodrug,\n                    <jats:bold>Sanger\u2010Naph\u2010CPT<\/jats:bold>\n                    , which enables light\u2010controlled camptothecin (CPT) release in cancer cells via 400\u2009nm light. Integrating a naphthalimide fluorescent reporter, the system allows real\u2010time monitoring of drug release kinetics. The photocaged prodrug was characterized by HPLC\/HRMS, confirming photodegradation under light. Confocal microscopy showed minimal baseline fluorescence in HeLa cells without irradiation, with robust fluorescence emerging postirradiation (intensity increasing with exposure time). MTT assays showed that, after light irradiation,\n                    <jats:bold>Sanger\u2010Naph\u2010CPT<\/jats:bold>\n                    exhibited dose\u2010dependent cytotoxicity comparable to free CPT, demonstrating restored activity of released CPT. This dual\u2010function platform combines light\u2010triggered therapeutic activity and fluorescence reporting, offering a modular design for photocontrolled prodrugs.\n                  <\/jats:p>","DOI":"10.1002\/cbic.70502","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T04:36:25Z","timestamp":1787891785000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sanger\u2019s Reagent\u2010Mediated Prodrug for Light\u2010Controlled Release of Camptothecin With Fluorescent Indication"],"prefix":"10.1002","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-8910-2234","authenticated-orcid":false,"given":"Zhennan","family":"Jiang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Materials\u2010Oriented Chemical Engineering College of Chemical Engineering Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) Nanjing Tech University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gengwen","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Materials\u2010Oriented Chemical Engineering College of Chemical Engineering Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) Nanjing Tech University  Nanjing China"},{"name":"Hematology Product Development Department Shenzhen Mindray Bio\u2010Medical Electronics Co. Ltd.  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Materials\u2010Oriented Chemical Engineering College of Chemical Engineering Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) Nanjing Tech University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Materials\u2010Oriented Chemical Engineering College of Chemical Engineering Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) Nanjing Tech University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0481-8531","authenticated-orcid":false,"given":"Sheng","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Materials\u2010Oriented Chemical Engineering College of Chemical Engineering Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) Nanjing Tech University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2493-2067","authenticated-orcid":false,"given":"Xiaoqiang","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Materials\u2010Oriented Chemical Engineering College of Chemical Engineering Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) Nanjing Tech University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","first-page":"122454","DOI":"10.1016\/j.biomaterials.2023.122454","article-title":"A Carbonic Anhydrase\u2010Targeted NIR\u2010II Fluorescent Cisplatin Theranostic Nanoparticle for Combined Therapy of Pancreatic Tumors","volume":"305","author":"Yang Y.","year":"2024","journal-title":"Biomaterials"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1186\/s12929-019-0583-0","article-title":"Host Immune Response to Anti\u2010Cancer Camptothecin Conjugated Cyclodextrin\u2010Based Polymers","volume":"26","author":"Chen Y. 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