{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T05:18:52Z","timestamp":1787894332329,"version":"build-2784847793"},"reference-count":47,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","award":["311771384"],"award-info":[{"award-number":["311771384"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","award":["444947649"],"award-info":[{"award-number":["444947649"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","award":["397427223"],"award-info":[{"award-number":["397427223"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2026,8,27]]},"abstract":"<jats:p>\n                    <jats:italic>\u03c9<\/jats:italic>\n                    \u2010Hydroxy and oxo acids are versatile platform chemicals, accessible via enzymes of the iron\u2010containing alcohol dehydrogenase (FeADH) superfamily. Compared with well\u2010characterized zinc\u2010dependent ADHs, FeADHs producing such compounds remain poorly defined regarding assignment to genetic locus, biochemical and biophysical properties, and metal specificity. Here, we uncover two FeADHs from\n                    <jats:italic>Clostridium viride<\/jats:italic>\n                    : a highly selective 4\u2010hydroxybutyrate dehydrogenase and a broad\u2010spectrum 5\u2010hydroxyvalerate dehydrogenase highly active on C5\u2013C12 substrates, identified by purification from the native organism. Chemical synthesis of 5\u2010oxovalerate enabled determination of kinetics for the physiological direction (\n                    <jats:italic>V<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    \u2009=\u20090.47\u2009\u00b1\u20090.05\u2009kU\/mg,\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    \u2009=\u20090.047\u2009\u00b1\u20090.011\u2009mM). These values compare favorably with those of the oxidation (\n                    <jats:italic>V<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    \u2009=\u20090.13\u2009\u00b1\u20090.02\u2009kU\/mg,\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    \u2009=\u20090.42\u2009\u00b1\u20090.08\u2009mM). We report the first crystal structure of an FeADH in complex with both substrate and NADH at 1.73\u2009\u00c5 resolution. This enables identification of active\u2010site iron and catalytic residues, providing insight into substrate recognition. High iron occupancy in the nonreconstituted enzymes, together with M\u00f6ssbauer and electron paramagnetic resonance (EPR)\u00a0spectroscopic identification of high\u2010spin Fe\n                    <jats:sup>2+<\/jats:sup>\n                    , establishes iron as the native cofactor. These findings highlight the potential of\n                    <jats:italic>C. viride<\/jats:italic>\n                    FeADHs as attractive biocatalysts for accessing sustainable solvents, polymers, and fine chemicals via\n                    <jats:italic>\u03c9<\/jats:italic>\n                    \u2010functionalized acids.\n                  <\/jats:p>","DOI":"10.1002\/cbic.70511","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:14:31Z","timestamp":1787310871000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["\u03c9\u2010Hydroxyacid Dehydrogenases From\n                    <i>Clostridium viride<\/i>\n                    : Iron\u2010Containing Biocatalysts With Biotechnological Potential"],"prefix":"10.1002","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9897-5700","authenticated-orcid":false,"given":"Tarik","family":"Begic","sequence":"first","affiliation":[{"name":"Biochemistry Department of Chemistry RPTU University Kaiserslautern\u2010Landau  Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9969-2958","authenticated-orcid":false,"given":"Julia","family":"Hill","sequence":"additional","affiliation":[{"name":"Biochemistry Department of Chemistry RPTU University Kaiserslautern\u2010Landau  Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3324-1064","authenticated-orcid":false,"given":"Jan L.","family":"Stitzel","sequence":"additional","affiliation":[{"name":"Biochemistry Department of Chemistry RPTU University Kaiserslautern\u2010Landau  Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1839-0412","authenticated-orcid":false,"given":"Lukas","family":"Knauer","sequence":"additional","affiliation":[{"name":"Department of Physics RPTU University Kaiserslautern\u2010Landau  Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3162-1032","authenticated-orcid":false,"given":"Volker","family":"Sch\u00fcnemann","sequence":"additional","affiliation":[{"name":"Department of Physics RPTU University Kaiserslautern\u2010Landau  Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6737-7203","authenticated-orcid":false,"given":"Markus","family":"R\u00e4schle","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics RPTU University Kaiserslautern\u2010Landau  Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9250-000X","authenticated-orcid":false,"given":"Berta M.","family":"Martins","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Biologie Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1509-6370","authenticated-orcid":false,"given":"Antonio J.","family":"Pierik","sequence":"additional","affiliation":[{"name":"Biochemistry Department of Chemistry RPTU University Kaiserslautern\u2010Landau  Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010008\u20108589\u2010x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432\u20101033.1991.tb16215.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014\u20105793(83)80517\u20101"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0166851"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2011.01.045"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae1010"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00775\u2010017\u20101439\u20102"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010013\u20104725\u20100"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.027201\u20100"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/biotech15010006"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2015.10.024"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2535786123"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0711093105"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/prep.1995.1026"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2020.140376"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.11.5671\u20105684.2002"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssuschemeng.0c08118"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2024.06.008"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)48036-2"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00282102"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002030000195"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.4056\/sigs.5068949"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac976"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2024.464908"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1096"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.9b03739"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432\u20101033.1989.tb14658.x"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcatb.2010.04.010"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.16603"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986610790963564"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167\u20104838(82)90206\u20100"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006\u20103495(89)82696\u20107"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1088\/0034\u20104885\/63\/3\/202"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432\u20101033.1989.tb14621.x"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2022.108504"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41929\u2010025\u201001299\u20105"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432\u20101033.1992.tb16976.x"},{"key":"e_1_2_9_39_1","volume-title":"Data 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