{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T05:18:13Z","timestamp":1787894293876,"version":"build-2784847793"},"reference-count":38,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19H05633"],"award-info":[{"award-number":["19H05633"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22H04743"],"award-info":[{"award-number":["22H04743"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24H02267"],"award-info":[{"award-number":["24H02267"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24H00489"],"award-info":[{"award-number":["24H00489"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21H00273"],"award-info":[{"award-number":["21H00273"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23H02101"],"award-info":[{"award-number":["23H02101"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["26H01674"],"award-info":[{"award-number":["26H01674"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["26K01641"],"award-info":[{"award-number":["26K01641"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["JPMJCR25T3"],"award-info":[{"award-number":["JPMJCR25T3"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2026,8,27]]},"abstract":"<jats:p>\n                    Fluorescent proteins (FPs) that are pH\u2010sensitive play a crucial role in investigating pH\u2010related cellular processes, such as endocytosis and exocytosis. Existing pH\u2010sensitive FPs generated from\n                    <jats:italic>Aequorea victoria<\/jats:italic>\n                    green fluorescent protein (GFP), such as superecliptic pHluorin (SEP) and Lime, have been widely employed to study these processes, but suffer from low photostability. Here, we report the development and characteristics of serapH, a genetically encodable pH biosensor with improved photostability compared to GFP analogues, which we generated using mStayGold as a scaffold. To aid in the development of serapH, we developed a method for screening pH\u2010sensitive FP variants by directly evaluating both brightness and pH sensitivity in bacterial colonies on agar. This significantly increased the number of colonies that could be screened per round and reduced the time needed per round. The photostability of serapH should improve spatiotemporal resolution by increasing tolerance to higher excitation intensities and longer imaging durations, thereby expanding the range of applications of pH\u2010sensitive FPs.\n                  <\/jats:p>","DOI":"10.1002\/cbic.70512","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T13:01:41Z","timestamp":1787749301000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of a Photostable pH Biosensor Based on mStayGold"],"prefix":"10.1002","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-7121-9364","authenticated-orcid":false,"given":"Michael","family":"Chang","sequence":"first","affiliation":[{"name":"Department of Chemistry Graduate School of Science The University of Tokyo  Bunkyo\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6955-4858","authenticated-orcid":false,"given":"Lilly","family":"Nash","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences The Royal Veterinary College  London UK"},{"name":"Department of Molecular &amp; Biomedical Sciences St. George\u2019s School of Health and \u00ad\u00adMedical Sciences City St. George\u2019s University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1205-9689","authenticated-orcid":false,"given":"Ruben","family":"Bierings","sequence":"additional","affiliation":[{"name":"Department of Hematology Erasmus University Medical Center  Rotterdam Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7925-6636","authenticated-orcid":false,"given":"Kei","family":"Takahashi\u2010Yamashiro","sequence":"additional","affiliation":[{"name":"Department of Chemistry Graduate School of Science The University of Tokyo  Bunkyo\u2010ku Tokyo Japan"},{"name":"Core Research for Evolutional Science and Technology (CREST) Japan Science and Technology Agency (JST)  Chiyoda\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3425-3589","authenticated-orcid":false,"given":"Takuya","family":"Terai","sequence":"additional","affiliation":[{"name":"Department of Chemistry Graduate School of Science The University of Tokyo  Bunkyo\u2010ku Tokyo Japan"},{"name":"Core Research for Evolutional Science and Technology (CREST) Japan Science and Technology Agency (JST)  Chiyoda\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6248-7278","authenticated-orcid":false,"given":"Tom","family":"Carter","sequence":"additional","affiliation":[{"name":"Department of Molecular &amp; Biomedical Sciences St. George\u2019s School of Health and \u00ad\u00adMedical Sciences City St. George\u2019s University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0604-092X","authenticated-orcid":false,"given":"Robert E.","family":"Campbell","sequence":"additional","affiliation":[{"name":"Department of Chemistry Graduate School of Science The University of Tokyo  Bunkyo\u2010ku Tokyo Japan"},{"name":"Core Research for Evolutional Science and Technology (CREST) Japan Science and Technology Agency (JST)  Chiyoda\u2010ku Tokyo Japan"},{"name":"CERVO Brain Research Center and Department of Biochemistry Microbiology and Bioinformatics Universit\u00e9 Laval  Qu\u00e9bec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8595-0827","authenticated-orcid":false,"given":"Kelvin K.","family":"Tsao","sequence":"additional","affiliation":[{"name":"Department of Chemistry Graduate School of Science The University of Tokyo  Bunkyo\u2010ku Tokyo Japan"},{"name":"Global Standard Science Education Graduate School of Science The University of Tokyo  Bunkyo\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2820"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(00)76468-X"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtha.2024.01.015"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2025.1624487"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-020-00718-x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.8b00333"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1339518"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/28190"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1042-04.2004"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1312982110"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.201411169"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-40557-y"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2024.114186"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M412547200"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2024027935"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-05-429936"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.3c00484"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.056218"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3791\/55466"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)00975-3"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(98)77870-1"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20221413"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac403585p"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abb7438"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9LC00779B"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4SD00138A"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.3c01573"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-022-01278-2"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-023-02085-6"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-024-02203-y"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/sb300037w"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0398-258"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2007.132993"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2012.01336.x"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0509378103"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/cn400012b"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1037"}],"container-title":["ChemBioChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.70512","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbic.70512","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.70512","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T04:37:43Z","timestamp":1787891863000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cbic.70512"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,26]]},"references-count":38,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2026,8,27]]}},"alternative-id":["10.1002\/cbic.70512"],"URL":"https:\/\/doi.org\/10.1002\/cbic.70512","archive":["Portico"],"relation":{},"ISSN":["1439-4227","1439-7633"],"issn-type":[{"value":"1439-4227","type":"print"},{"value":"1439-7633","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,26]]},"assertion":[{"value":"2026-03-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70512"}}