{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T05:19:20Z","timestamp":1787894360070,"version":"build-2784847793"},"reference-count":29,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T00:00:00Z","timestamp":1787270400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22137004"],"award-info":[{"award-number":["22137004"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82430109"],"award-info":[{"award-number":["82430109"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22541704"],"award-info":[{"award-number":["22541704"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC3605400"],"award-info":[{"award-number":["2023YFC3605400"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2026,8,27]]},"abstract":"<jats:p>\n                    Extracellular vesicle (EV)\u2010mediated protein transport has been recognized as a crucial mechanism for intercellular communication and interaction. Proximity labeling has emerged as a promising approach to study the dynamic protein interactions in cellular activity, but tracking extracellular protein transport pathways remains to be further investigated. Here, we employ Ru(bpy)\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>2+<\/jats:sup>\n                    (RU) to construct a spatiotemporally resolved Photocatalytic Extracellular Vesicles protein Tracking (PhoEVT) system. Upon 450\u2009nm irradiation, RU generates singlet oxygen to trigger the biotinylation of EV\u2010associated proteins with high efficiency. The photocatalyst was used as a nongenetic alternative to enzymatic approaches for singlet oxygen generation upon 450\u2009nm photoirradiation during EV purification, which allowed the subsequent labeling of nearby accessible proteins with the biotin\u2010aniline probe. Using spintip streptavidin\u2010mediated affinity purification followed by mass spectrometry, we tracked EV\u2010associated protein signals and detected EV\u2010derived proteins in recipient cells. Based on the PhoEVT system, we demonstrated the uptake dynamics of EV\u2010associated proteins in recipient cells and performed unbiased proteomic profiling of detected EV\u2010derived proteins. Furthermore, we monitored the presence and temporal changes of EV\u2010associated proteins during the interaction of tumor cell\u2010derived EVs with immune cells, providing an effective tool to better investigate the dynamics of EV\u2010associated proteins.\n                  <\/jats:p>","DOI":"10.1002\/cbic.70515","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:29:38Z","timestamp":1787311778000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Spatiotemporally Resolved Tracking of Extracellular Vesicle Cargo via Photocatalytic Extracellular Vesicles Protein Tracking (PhoEVT)"],"prefix":"10.1002","volume":"27","author":[{"given":"Shuai\u2010Ting","family":"Yan","sequence":"first","affiliation":[{"name":"State Key Laboratory of Membrane Biology School of Pharmaceutical Sciences Tsinghua\u2010Peking Center for Life Sciences Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education) Tsinghua University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Ting","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences  Tsinghua\u2010Peking Center for Life Sciences State Key Laboratory of Membrane Biology MOE Key Laboratory of Bioorganic Phosphorus Chemistry &amp; Chemical Biology Beijing Frontier Research Center for Biological Structure Tsinghua University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3362-3952","authenticated-orcid":false,"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Membrane Biology School of Pharmaceutical Sciences Tsinghua\u2010Peking Center for Life Sciences Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education) Tsinghua University  Beijing 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