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These carbon\u2010modified sugars have received only limited attention, and systematic investigations on their ability to act as substrates of glycosyl hydrolases have not been reported. Here, C6\u2010substituted \u03b2\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010galactosides are synthesized and evaluated as putative substrates of \u03b2\u2010glycosyl hydrolases. The results indicated that the size of the group at C6\u2014methyl vs. di\u2010methyl vs. phenyl\u2014attenuated their ability to act as substrates. The C6\u2010methyl derivative was a weak substrate, but the C6\u2010dimethyl and C6\u2010phenyl compounds were not. The new \u03b2\u2010galactosides can be leveraged as tool compounds in the search for glycosidases with novel activity and as starting points for enzyme\u2010engineering campaigns.\n                  <\/jats:p>","DOI":"10.1002\/cbic.70524","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T18:52:20Z","timestamp":1788202340000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unnatural C6\u2010Substituted\n                    <scp>d<\/scp>\n                    \u2010Galactosides as Attenuated Substrates of Glycosyl Hydrolases"],"prefix":"10.1002","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6830-1155","authenticated-orcid":false,"given":"Andrey Yu.","family":"Plodukhin","sequence":"first","affiliation":[{"name":"Department of Chemistry University of Connecticut  Storrs Connecticut 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