{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T07:49:23Z","timestamp":1791964163758,"version":"4.3.4"},"reference-count":30,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T00:00:00Z","timestamp":1789344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T00:00:00Z","timestamp":1789344000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004189","name":"Max-Planck-Gesellschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004189","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010740","name":"Asahi Kasei Pharma Corporation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010740","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2026,9,14]]},"abstract":"<jats:p>\n                    In the hydrogenotrophic methanogens cultivated under nickel\u2010sufficient standard medium (~5 \u00b5M Ni\n                    <jats:sup>2+<\/jats:sup>\n                    ), two cytosolic [NiFe]\u2010hydrogenases, F\n                    <jats:sub>420<\/jats:sub>\n                    \u2010reducing hydrogenase (Frh) and heterodisulfide reductase (Hdr)\u2010associating hydrogenase (Mvh), mainly provide electrons to the methanogenic pathway. In\n                    <jats:italic>Methanothermobacter marburgensis<\/jats:italic>\n                    under strictly nickel\u2010limited conditions (&lt; 50\u2009nM Ni\n                    <jats:sup>2+<\/jats:sup>\n                    ), which are encountered in certain natural environments, Frh and Mvh are strongly downregulated. Under these conditions, a coupled reaction with [Fe]\u2010hydrogenase (Hmd) and F\n                    <jats:sub>420<\/jats:sub>\n                    \u2010dependent methylene\u2010tetrahydromethanopterin dehydrogenase (Mtd) substitutes for the function of Frh, where F\n                    <jats:sub>420<\/jats:sub>\n                    \u2010dependent electron\u2010donating protein (Elp) complexes with Hdr and donates electrons from the reduced form of F\n                    <jats:sub>420<\/jats:sub>\n                    to Hdr. Thus, Hmd mainly provides electrons to methanogenesis in nickel\u2010limited environments. In this study, to evaluate the potential of isocyanides as an Hmd\u2010specific chemical tool to probe this pathway, we determined their inhibitory effects on the H\n                    <jats:sub>2<\/jats:sub>\n                    \u2010dependent F\n                    <jats:sub>420<\/jats:sub>\n                    \u2010reducing activity in the cell extract and on the in vitro methanogenesis reaction in a cell extract and in a cell suspension. Isocyanides specifically inhibited the activities of the samples from nickel\u2010limited cells. The inhibitory effect on the cell suspension was strengthened by the use of a hydrophobic isocyanide. These results demonstrate their potential as a chemical tool for identifying in situ Hmd\u2010dependent methanogenesis activity in natural environmental samples.\n                  <\/jats:p>","DOI":"10.1002\/cbic.70535","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T08:09:32Z","timestamp":1789373372000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isocyanides as a Chemical Tool for Identifying [Fe]\u2010Hydrogenase\u2010Dependent Methanogenesis"],"prefix":"10.1002","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-1762-6475","authenticated-orcid":false,"given":"Shunsuke","family":"Nomura","sequence":"first","affiliation":[{"name":"Microbial Protein Structure Group, Max Planck Institute for Terrestrial Microbiology  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1872-8705","authenticated-orcid":false,"given":"Seigo","family":"Shima","sequence":"additional","affiliation":[{"name":"Microbial Protein Structure Group, Max Planck Institute for Terrestrial Microbiology  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1931"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010micro\u2010011720\u2010122807"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.030508.152103"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41570\u2010020\u20100167\u20102"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432\u20101033.1992.tb17215.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202300330"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2010.12.014"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201305089"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41929\u2010024\u201001262\u2010w"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1213193109"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010025\u201009229\u2010y"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050562"},{"key":"e_1_2_9_14_1","first-page":"375","article-title":"Nickel: A Review of Its Sources and Environmental Toxicology","volume":"15","author":"Cempel M.","year":"2006","journal-title":"Polish Journal of Environmental Studies"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00061"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07858"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmars.2020.00105"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203\u2010003\u20100577\u20109"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1016761108"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abg5550"},{"key":"e_1_2_9_21_1","volume-title":"Diversity of Electron\u2010Bifurcating CO2\u2010Fixing Supercomplexes in Methanogens","author":"Segundo\u2010Acosta P. S.","year":"2026"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.17115"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010026\u201010744\u20109"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1003653107"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.59.070190.002035"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201813465"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076\u20106879(01)31069\u20108"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978\u20100\u201012\u2010385112\u20103.00007\u2010X"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432\u20101033.1988.tb13941.x"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.1.60"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021\u20109258(18)67912\u20108"}],"container-title":["ChemBioChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.70535","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbic.70535","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.70535","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T07:37:22Z","timestamp":1791963442000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cbic.70535"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,14]]},"references-count":30,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2026,9,14]]}},"alternative-id":["10.1002\/cbic.70535"],"URL":"https:\/\/doi.org\/10.1002\/cbic.70535","archive":["Portico"],"relation":{},"ISSN":["1439-4227","1439-7633"],"issn-type":[{"value":"1439-4227","type":"print"},{"value":"1439-7633","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,14]]},"assertion":[{"value":"2026-06-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70535"}}