{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T07:49:04Z","timestamp":1791964144526,"version":"4.3.4"},"reference-count":44,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2026,10,14]]},"abstract":"<jats:p>\n                    Antibiotic resistance caused by metallo\u2010\u03b2\u2010lactamases (M\u03b2Ls) is a global public health threat. In this work, we first directly investigated the binding of Zn\n                    <jats:sup>2+<\/jats:sup>\n                    to apo\u2010M\u03b2Ls through isothermal titration calorimetry. It was found that carbapenem and cephamycin resistance metallo\u2010\u03b2\u2010lactamase (CcrA), New Delhi metallo\u2010\u03b2\u2010lactamase\u20101 (NDM\u20101) and\n                    <jats:italic>Stenotrophomonas maltophilia<\/jats:italic>\n                    Metallo\u2010\u03b2\u2010lactamase\u20101(L1) bind Zn\n                    <jats:sup>2+<\/jats:sup>\n                    in a sequential mode, while imipenemase\u20101 (IMP\u20101) and Verona Integron\u2010encoded metallo\u2010\u03b2\u2010lactamase\u20102 (VIM\u20102) bind Zn\n                    <jats:sup>2+<\/jats:sup>\n                    in a mode of varying levels of cooperativity, in which the mononuclear species was not detectable, and also, the mode was verified through the titration of Co\n                    <jats:sup>2+<\/jats:sup>\n                    into apo\u2010VIM\u20102 monitored by UV\u2013vis. The thermodynamic and kinetic parameters revealed the binding of Zn\n                    <jats:sup>2+<\/jats:sup>\n                    to the active sites was independent of stoichiometry. Analysis of the parameters determined suggested that the binding of B1 and B3\u2009M\u03b2Ls to Zn\n                    <jats:sup>2+<\/jats:sup>\n                    depends on the binding affinity and\/or Gibbs free energy change of Zn\n                    <jats:sub>1<\/jats:sub>\n                    and Zn\n                    <jats:sub>2<\/jats:sub>\n                    sites of the enzymes, and two metal sites have almost equal contributions for full activity of the M\u03b2Ls. The information gained in this work is helpful for the further mechanism studies and the development of the broad\u2010spectrum M\u03b2L inhibitors.\n                  <\/jats:p>","DOI":"10.1002\/cbic.70563","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T07:03:28Z","timestamp":1791875008000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Binding of Zinc(II) to Apo\u2010Metallo\u2010\u03b2\u2010Lactamases: Characterization by Isothermal Titration Calorimetry"],"prefix":"10.1002","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9632-8278","authenticated-orcid":false,"given":"Le","family":"Zhai","sequence":"first","affiliation":[{"name":"Engineering Research Center for Titanium Based Functional Materials and Devices in Universities of Shaanxi Province College of Chemistry and Materials Engineering Baoji University of Arts and Sciences  Baoji P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiazhu","family":"Chigan","sequence":"additional","affiliation":[{"name":"Anti\u2010Drug Technology Center of Shaanxi Provincial Public Security Department National Anti\u2010drug Laboratory Shaanxi Regional Center  Xi\u2019an P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Ming","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education Chemical Biology Innovation Laboratory College of Chemistry and Materials Science Northwest University  Xi\u2019an P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Wang","sequence":"additional","affiliation":[{"name":"Engineering Research Center for Titanium Based Functional Materials and Devices in Universities of Shaanxi Province College of Chemistry and Materials Engineering Baoji University of Arts and Sciences  Baoji P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4910-4380","authenticated-orcid":false,"given":"Hua\u2010Ping","family":"Xie","sequence":"additional","affiliation":[{"name":"Engineering Research Center for Titanium Based Functional Materials and Devices in Universities of Shaanxi Province College of Chemistry and Materials Engineering Baoji University of Arts and Sciences  Baoji P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5140-3760","authenticated-orcid":false,"given":"Yao","family":"Zhao","sequence":"additional","affiliation":[{"name":"Engineering Research Center for Titanium Based Functional Materials and Devices in Universities of Shaanxi Province College of Chemistry and Materials Engineering Baoji University of Arts and Sciences  Baoji P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Qiang","family":"Wu","sequence":"additional","affiliation":[{"name":"Engineering Research Center for Titanium Based Functional Materials and Devices in Universities of Shaanxi Province College of Chemistry and Materials Engineering Baoji University of Arts and Sciences  Baoji P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Xue","family":"Wang","sequence":"additional","affiliation":[{"name":"Engineering Research Center for Titanium Based Functional Materials and Devices in Universities of Shaanxi Province College of Chemistry and Materials Engineering Baoji University of Arts and Sciences  Baoji P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei\u2010Xing","family":"Zhao","sequence":"additional","affiliation":[{"name":"Engineering Research Center for Titanium Based Functional Materials and Devices in Universities of Shaanxi Province College of Chemistry and Materials Engineering Baoji University of Arts and Sciences  Baoji P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2560-9659","authenticated-orcid":false,"given":"Ke\u2010Wu","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education Chemical Biology Innovation Laboratory College of Chemistry and Materials Science Northwest University  Xi\u2019an P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473\u20103099(20)30868\u20109"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0PY01751E"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010017\u201000601\u20109"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.1c00138"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abo7793"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi990356r"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460\u20102075.1995.tb00174.x"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M301062200"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3109\/1040841X.2011.559944"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.7.1584"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.2.436"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.15.4611\u20104617.1991"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.7.2330\u20102333.2003"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.2148"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.7.1878\u20101886.2000"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.6.1538\u20101543.2000"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05654\u201011"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00774\u201009"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0924\u20108579(10)70004\u20102"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja303579d"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja410376s"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi051105n"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi500916y"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi047463s"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/ma9917139"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tca.2012.04.003"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tca.2013.01.015"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10973\u2010011\u20101979\u20106"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969\u20102126(96)00089\u20105"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20090434"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2021.105138"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2021.116128"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41429\u2010021\u201000440\u20103"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2022.105910"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2021.105474"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmr.v17:6"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4SC00601A"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi200839h"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00775\u2010006\u20100083\u2010z"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00075a046"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.35.22402"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi300056y"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2004.04.017"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.4.921"}],"container-title":["ChemBioChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.70563","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbic.70563","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.70563","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T07:34:10Z","timestamp":1791963250000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cbic.70563"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,13]]},"references-count":44,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2026,10,14]]}},"alternative-id":["10.1002\/cbic.70563"],"URL":"https:\/\/doi.org\/10.1002\/cbic.70563","archive":["Portico"],"relation":{},"ISSN":["1439-4227","1439-7633"],"issn-type":[{"value":"1439-4227","type":"print"},{"value":"1439-7633","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,13]]},"assertion":[{"value":"2026-03-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70563"}}