{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T11:11:38Z","timestamp":1789211498976,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,3,15]],"date-time":"2013-03-15T00:00:00Z","timestamp":1363305600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Gene therapy and nerve stem cells isolated from the developing human enteric nervous system (ENS) are significant. They may open the route for the cell therapy of Hirschsprung's disease (HD). We have constructed the recombinant adenovirus\u2010carrying glial cell line\u2010derived neurotrophic factor (GDNF) and endothelin receptor B (EDNRB) gene, and investigated the exosomatic coexpression in neural stem cells. The recombinant adenovirus Ad\u2010GE coexpressing GDNF and EDNRB gene was constructed by the AdEasy system and confirmed by the reverse transcription polymerase chain reaction (RT\u2010PCR) method. Expression of exogenous genes in neural stem cells after transfection was confirmed by the flow cytometry and real\u2010time fluorescence quantitative PCR. Fragments of pAd Track\u2010CMV\u2010GE were consistent with GDNF and EDNRB. The green fluorescence of the positive cells was followed by fluorescence microscopy at 24\u2009h after NSCs had been transfected, reaching a peak at 72\u2009h after transfection. Flow cytometry showed that the efficiency of transfection was 15.0, 23.6, and 25.4% at 24, 48 and 72\u2009h respectively. Real\u2010time fluorescence quantitative PCR showed the expression levels of mRNA of GDNF and EDNRB in 48 and 72\u2009h groups were obviously higher than that in 24 and 96\u2009h groups. Recombinant adenovirus carrying GDNF and EDNRB genes are coexpressed in neural stem cells, which may offer the possibility of a novel approach to local combination gene therapy for Hirschsprung's disease.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10060","type":"journal-article","created":{"date-parts":[[2013,8,6]],"date-time":"2013-08-06T09:54:35Z","timestamp":1375782875000},"page":"458-463","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Coexpression of recombinant adenovirus carrying GDNF and EDNRB genes in neural stem cells in vitro"],"prefix":"10.1002","volume":"37","author":[{"given":"Nian\u2010Feng","family":"Sun","sequence":"first","affiliation":[{"name":"Department of General Surgery Qilu Hospital of Shandong University Jinan 250012 China"},{"name":"Department of the Surgery Affiliated Jinan Central Hospital of Shandong University Jinan 250013 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