{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T04:48:30Z","timestamp":1773204510649,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,4,8]],"date-time":"2013-04-08T00:00:00Z","timestamp":1365379200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>It has been demonstrated that aldosterone (ALD) plays a direct profibrotic role in the kidney but the underlying mechanism remains unclear. We examined the role of Rho kinase signal pathway in epithelial\u2013mesenchymal transition (EMT) process and extracellular matirx (ECM) synthesis induced by ALD in human renal proximal tubular epithelial (HK\u20102) cells in vitro. Rho kinase and collagen I, III protein expressions were detected by ELISA. E\u2010cadherin, \u03b1\u2010smooth muscle actin (SMA), collagen_I and collagen III mRNA expressions were detected by real time PCR. E\u2010cadherin, and \u03b1\u2010SMA protein expressions were measured by Western blot. Our results showed that ALD could significantly activate the Rho kinase in HK\u20102 cells, while in the presence of mineralocorticoid receptor (MR) antagonist eplerenone and Rho kinase inhibitor Y27632, the Rho kinase protein expression were almost completely prevented. Exposure of HK\u20102 cells to ALD for 48\u2009h induced EMT as evidenced by loss of E\u2010cadherin, and de novo expression of \u03b1\u2010SMA. The EMT was completely blocked by eplerenone and Y27632. Meanwhile, ALD could significantly increase the mRNA and protein expressions of collagen I, III in HK\u20102 cells when compared with the control group, while eplerenone and Y27632 could almost reverse these effects. These observations suggest that ALD can activate Rho kinase pathway and Rho kinase pathway is likely responsible for the profibrotic actions of ALD in renal proximal tubular epithelial cells via inducing EMT and ECM excretion.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10082","type":"journal-article","created":{"date-parts":[[2013,8,6]],"date-time":"2013-08-06T09:54:05Z","timestamp":1375782845000},"page":"725-730","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Rho kinase pathway is likely responsible for the profibrotic actions of aldosterone in renal epithelial cells via inducing epithelial\u2013mesenchymal transition and extracellular matrix excretion"],"prefix":"10.1002","volume":"37","author":[{"given":"Jiali","family":"Wei","sequence":"first","affiliation":[{"name":"Department of Nephrology Hainan General Hospital Haikou Hainan 570311 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