{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T11:48:54Z","timestamp":1793015334367,"version":"4.5.0"},"reference-count":53,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,5,28]],"date-time":"2013-05-28T00:00:00Z","timestamp":1369699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      We have examined the effect of oleic acid (OA) concentrations and incubation time, along with chicken serum (CS), on adipogenic differentiation and expression of adipogenic transcripts in hen preadipocytes. Preadipocytes were treated with (i) an adipogenic cocktail (DMI) containing 500\u2009nM dexamethasone, 0.5\u2009mM 3\u2010isobutyl\u20101\u2010methylxanthine and 20\u2009\u00b5g\/mL insulin alone and DMI\u2009+\u200975, 150, 300 or 600\u2009\u00b5M OA for 48\u2009h; (ii) DMI\u2009+\u2009300\u2009\u00b5M OA (DMIOA) for 6, 12, 24 or 48\u2009h; and (iii) foetal bovine serum (FBS), CS, DMI\u2009+\u2009FBS, DMI\u2009+\u2009CS, DMIOA\u2009+\u2009FBS and DMIOA\u2009+\u2009CS. While FABP4 was significantly expressed with increasing concentrations of OA, the expression of C\/EBP\u03b2, LEPR and FAS were unchanged compared with the control. PPAR\u03b32 expression was unchanged across all time\u2010points. A significantly higher level of C\/EBP\u03b1 was measured at 48\u2009h, but the levels of C\/EBP\u03b2 increased after 12\u2009h. Levels of FABP4 significantly increased with the time of incubation after 12\u2009h, but that of LPL was reduced (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05) at 6, 24 and 48\u2009h. FABP4 was highly expressed in cells treated with CS, DMI\u2009+\u2009CS and DMIOA\u2009+\u2009CS compared to cells treated with FBS, DMI\u2009+\u2009FBS and DMIOA\u2009+\u2009FBS. In conclusion, increased concentrations of OA and incubation time increases lipid accumulation; FABP4 and C\/EBP\u03b2 are potential transcription factors regulating OA induced adipogenesis of fat cells obtained from laying hen. CS is a potent inducer of adipogenic differentiation in hen preadipocytes.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cbin.10122","type":"journal-article","created":{"date-parts":[[2013,8,6]],"date-time":"2013-08-06T05:55:19Z","timestamp":1375768519000},"page":"961-971","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Effects of oleic acid and chicken serum on the expression of adipogenic transcription factors and adipogenic differentiation in hen preadipocytes"],"prefix":"10.1002","volume":"37","author":[{"given":"Alemu","family":"Regassa","sequence":"first","affiliation":[{"name":"Department of Animal Science University of Manitoba Winnipeg Manitoba R3T 2N2 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Woo Kyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Animal Science University of Manitoba Winnipeg Manitoba R3T 2N2 Canada"},{"name":"Department of Poultry Science University of Georgia Athens Georgia 30602 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,5,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1016\/S0022-2275(20)41913-3","article-title":"Regulation of adipose cell differentiation. II. Kinetics of induction of the AP2 gene by fatty acids and modulation by dexamethasone","volume":"32","author":"Amri EZ","year":"1991","journal-title":"J Lipid Res"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.5.2367"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1042\/bj2380115","article-title":"Adipose cell differentiation: evidence for a two\u2010step process in the polyamine\u2010dependent Ob1754 clonal line","volume":"238","author":"Amri EZ","year":"1986","journal-title":"J Biochem"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(86)91165-4"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(1998)72:30\/31 <73::AID-JCB11>3.0.CO;2-L"},{"key":"e_1_2_7_7_1","first-page":"109","article-title":"The effect of sera on chicken adipocyte differentiation in culture","volume":"38","author":"ChaoHua W","year":"2009","journal-title":"J Chin Soc Anim Sci"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(97)04599-0"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03194597"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nu.14.070194.000531"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.shk.0000235087.45798.93"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-005-3857-7"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-2863(00)00136-4"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.2741\/Goldberg"},{"key":"e_1_2_7_15_1","first-page":"664","article-title":"Comparison of the adipo conversion of preadipocytes derived from lean and obese zucker rats in serum\u2010free cultures","volume":"19","author":"Gregoire FM","year":"1995","journal-title":"Int J Obes Relat Metab Disord"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.22.10930"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100797200"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00465.2005"},{"key":"e_1_2_7_19_1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1042\/bj2890141","article-title":"Cloning of alpha 2 chain of type vi collagen and expression during mouse development","volume":"289","author":"Ibrahimi A","year":"1993","journal-title":"J Biochem"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2004-0395"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.9.4318"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2121-9-60"},{"key":"e_1_2_7_23_1","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1001\/jama.1994.03520030047027","article-title":"Increasing prevalence of overweight among US adults. The National Health and Nutrition Examination Surveys 1960 to 1991","volume":"272","author":"Kuczmarski RJ","year":"1994","journal-title":"JAMA"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.64.070195.002021"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-2760(01)00517-3"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1503\/cmaj.101522"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2008.07.002"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2005.04.013"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1080\/10409230591008189"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.90371.2008"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.3382\/japr.2010-00167"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.948702"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2008.06.010"},{"key":"e_1_2_7_34_1","unstructured":"SAS Institute(2008)SAS System for Statistical Analysis. Release 9.2. SAS Inst. Inc. Cary NC."},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1740-0929.2009.00639.x"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2008-00086"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2009.07.006"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2010-01161"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-7364-5-3-170"},{"key":"e_1_2_7_40_1","first-page":"34176","article-title":"Distinct transcriptional profiles of adipogenesis in vivo and in vitro","volume":"76","author":"Soukas A","year":"2001","journal-title":"J Biol Chem"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-052110-115718"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.04.176"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.90920.2008"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2007-00329"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.19.10261"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020550405578"},{"key":"e_1_2_7_47_1","doi-asserted-by":"crossref","first-page":"466S","DOI":"10.1093\/ajcn\/63.3.466","article-title":"Social and economic effects of body weight in the United States","volume":"63","author":"Wolf AM","year":"1996","journal-title":"Am J Clin Nutr"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2009.00709.x"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/79.6.892"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80467-0"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-925X-8-34"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2007-00220"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2011.11.013"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M510682200"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbin.10122","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.10122","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T11:15:07Z","timestamp":1696590907000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.10122"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5,28]]},"references-count":53,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2013,9]]}},"alternative-id":["10.1002\/cbin.10122"],"URL":"https:\/\/doi.org\/10.1002\/cbin.10122","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,28]]},"assertion":[{"value":"2013-02-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-04-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-05-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}