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To address the issue of overcoming instability and low transfection efficiency in vitro, the polyethylene glycol\u2013polyethyleneimine (PEG\u2013PEI) nanoparticle was used as non\u2010viral vector carrier for miR\u2010150 transfection, which is downregulated in chronic myeloid leukemia. PEG\u2013PEI [PEG<jats:sub>(550)3<\/jats:sub>\u2010g\u2010PEI<jats:sub>(1800)<\/jats:sub>]\/miRNA nanocomplexes were synthesised and characterised by particle size distribution (PSD), polydispersity index (PDI) and zeta potential, surface charge, their cytotoxicity, and transfection efficiency. Interaction with human leukemia cells (K\u2010562 and KU812) and control cells NCI\u2010BL2347 with them has been investigated. The transfection efficiency of PEG\u2013PEI\/miRNA at N\/P 26 rose 6.7\u2010fold above the control by qRT\u2010PCR. The size of homogenous nanocomplexes (PBI\u2009&lt;\u20090.5) was 160.8\u2009\u00b1\u200911\u2009nm. The data indicate that PEG\u2013PEI may be an encouraging non\u2010viral carrier for altering miRNA expression in the treatment of chronic myeloid leukemia, with many advantages such as relatively high miRNA transfection efficiency and low cytotoxicity.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10157","type":"journal-article","created":{"date-parts":[[2013,8,6]],"date-time":"2013-08-06T09:37:02Z","timestamp":1375781822000},"page":"1205-1214","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Design of polyethylene glycol\u2013polyethylenimine nanocomplexes as non\u2010viral carriers: mir\u2010150 delivery to chronic myeloid leukemia cells"],"prefix":"10.1002","volume":"37","author":[{"given":"\u00c7\u0131\u011f\u0131r","family":"Biray Avc\u0131","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Department of Medical Biology Ege University Bornova Izmir 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