{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T21:05:26Z","timestamp":1761599126813,"version":"3.37.3"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,11,15]],"date-time":"2013-11-15T00:00:00Z","timestamp":1384473600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003968","name":"Iran National Science Foundation","doi-asserted-by":"crossref","award":["8611973"],"award-info":[{"award-number":["8611973"]}],"id":[{"id":"10.13039\/501100003968","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>The inhibitory effects of acetazolamide on the growth and proliferation of epithelial breast cancer cells (T\u201047D) were investigated. Analysis of morphological changes indicated little apoptosis in T\u201047D cells incubated with acetazolamide, according to data from flow cytometry, DNA laddering, and expression of <jats:italic>AIF<\/jats:italic>. However, an increase in caspase\u20103 activity was detected in cells. This was concomitant with an increase in DFF45\/DFF40 ratio leading to inhibition of caspase\u20103 activity, DNA fragmentation and progression of apoptosis. Flow cytometry also confirmed that acetazolamide had no significant effect on cell cycle progression. These results are consistent with lack of change in the expression of cell cycle regulatory proteins p21, p27, cdc2 and cyclinD1. Increased expression of ATG5, p53 and DRAM, along with an increase in BCLN1\/Bcl\u20102 ratio, indicated that acetazolamide inhibited the proliferation of T\u201047D cells by inducing autophagy. Increased expression of PTEN, along with decreased expression of Akt1, also showed that acetazolamide treatment resulted in death inducing autophagy. Collectively the results indicate that autophagy is an adequate mechanism mediating the anti\u2010cancer effects of acetazolamide in T\u201047D cells through engagement of p53\/DRAM pathway and attenuation of Akt survival signalling.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10197","type":"journal-article","created":{"date-parts":[[2013,10,23]],"date-time":"2013-10-23T17:02:21Z","timestamp":1382547741000},"page":"228-238","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Acetazolamide triggers death inducing autophagy in T\u201047<scp>D<\/scp> breast cancer cells"],"prefix":"10.1002","volume":"38","author":[{"given":"Raziye","family":"Mohammadpour","sequence":"first","affiliation":[{"name":"Department of Cell and Molecular Biology School of Biology, College of Science University of Tehran Tehran 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