{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T05:00:16Z","timestamp":1768280416528,"version":"3.49.0"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,1,13]],"date-time":"2014-01-13T00:00:00Z","timestamp":1389571200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Human amniotic fluid stem cells (HAFSCs) have a high proliferative capacity and a good differentiation potential, and may thus be suitable for regenerative medicine. To date, urothelial differentiation mechanisms of HAFSCs are poorly understood. We have investigated the urothelial differentiation potential of HAFSCs so that they can be therapeutically applied to cure defective diseases of bladder. To induce the stem cell differentiation, HAFSCs were cultured in a bladder cancer\u2010derived conditioned medium. After 2 weeks of culture, HAFSCs began to express the urothelial lineage\u2010specific markers (UPII, CK8 and FGF10). Meanwhile, pluripotency markers (Oct\u20104, Sox\u20102 and Nanog) were downregulated at both RNA and protein levels in the differentiated HAFSCs. Immunocytochemistry data revealed that differentiated HAFSCs expressed urothelial markers of UPII and CK8. We have screened the receptor tyrosine kinase arrays with the differentiated HAFSCs. The screening showed that MuSK, Tie\u20101 and EphA4 receptor tyrosine kinases were upregulated, whereas EphA7 and FGF R1 kinases were downregulated in HAFSCs. The data suggest that HAFSCs can be an important urothelium cell source, which can be used for urinary tract engineering.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10232","type":"journal-article","created":{"date-parts":[[2013,12,23]],"date-time":"2013-12-23T13:56:11Z","timestamp":1387806971000},"page":"531-537","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Urothelial differentiation of human amniotic fluid stem cells by urothelium specific conditioned medium"],"prefix":"10.1002","volume":"38","author":[{"given":"Henry H.","family":"Kang","sequence":"first","affiliation":[{"name":"Hamilton College 198 College Hill Rd Clinton NY 13323, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia J.","family":"Kang","sequence":"additional","affiliation":[{"name":"Mylan School of Pharmacy Duquesne University 600 Forbes Ave Pittsburgh PA 15219, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyung\u2010Gyoo","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Pathology Children's Hospital in Los Angeles Los Angeles CA 90027, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyung S.","family":"Chung","sequence":"additional","affiliation":[{"name":"Division of Cancer Research and Training College of Medicine Charles Drew University of Medicine and Science 1731 East 120th Street Los Angeles CA, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,1,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-9-9"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/bmb\/ldr003"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)68438-9"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M201658200"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)60111-3"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1274"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2006.07.001"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.21.1.309"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81252-0"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2008.09.012"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2184.2007.00478.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018404927753"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2003.12.014"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0480"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1513\/pats.200801-008AW"},{"key":"e_1_2_8_17_1","first-page":"397","article-title":"Tie1, a receptor tyrosine kinase essential for vascular endothelial cell integrity, is not critical for the development of hematopoietic cells","volume":"12","author":"Rodewald H","year":"1996","journal-title":"Oncogene"},{"key":"e_1_2_8_18_1","first-page":"446","article-title":"Cryopreserved amniotic fluid\u2010derived cells: a lifelong autologous fetal stem cell source for heart valve tissue engineering","volume":"17","author":"Schmidt D","year":"2008","journal-title":"J Heart Valve Dis"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2009.09.015"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-007-0593-1"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2009.0625"},{"key":"e_1_2_8_22_1","doi-asserted-by":"crossref","first-page":"19170","DOI":"10.1016\/S0021-9258(17)30640-3","article-title":"Large scale purification and immunolocalization of bovine uroplakins I, II, and III. 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