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We report an efficient method to generate iPS cells from MEF (mouse embryonic fibroblasts) through folate\u2010depriviatoin, which was used to change the methylation of MEF. Without folate for 3 days, the induction efficiency is enhanced fivefold. Karyotype analysis showed that transient folate\u2010depriving treatment did not negatively affect properties of iPS cells; characterised iPS cells show normal karyotypes. 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