{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T06:26:37Z","timestamp":1789971997944,"version":"4.0.1"},"reference-count":35,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,4,24]],"date-time":"2014-04-24T00:00:00Z","timestamp":1398297600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>The effect of focal adhesion kinase (FAK) on suppressing scarring and the potential molecular mechanism underlying it has been investigated. Ten samples of human hypertrophic scars (HS) tissue cultured in vitro were transfected with FAK siRNA mediated by liposome. Quantitative real\u2010time PCR was used to detect the expression of integrin \u03b1, transforming growth factor\u2010\u03b2 (TGF\u2010\u03b2), FAK and \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA) after transfection. MTT assay was used as a measure of fibroblast proliferation. Flow cytometry and<jats:sup>3<\/jats:sup>H\u2010proincorporation technique gave measurements of the cell cycle and the quantity of collagen synthesis, respectively. Expression of FAK was effectively blocked, accompanied by decreasing expression of integrin \u03b1, TGF\u2010\u03b2 and \u03b1\u2010SMA in hypertrophic scars fibroblast (HSFB) cells. One to 4\u2009h after transfection with FAK siRNA, proliferation of HSFB cells was strongly inhibited (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01), reaching a maximum at 48\u2009h. The proportion of G<jats:sub>1<\/jats:sub>cells was higher and the proportion of the S and G<jats:sub>2<\/jats:sub>cells lower after transfection. The amount of collagen synthesis in HSFB cells decreased when HSFB cells were transfected for 48\u2009h. RNA interference targeting the FAK gene can block the two abnormal signal transduction pathways mediated by the integrin and TGF\u2010\u03b2 receptors that are responsible for hyperplasia and contracture of the scar, making FAK iRNA therapy a potentially effective approach in HS treatment.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10265","type":"journal-article","created":{"date-parts":[[2014,2,13]],"date-time":"2014-02-13T16:25:00Z","timestamp":1392308700000},"page":"803-808","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Focal adhesion kinase (FAK) siRNA inhibits human hypertrophic scar by suppressing integrin \u03b1, TGF\u2010\u03b2 and \u03b1\u2010SMA"],"prefix":"10.1002","volume":"38","author":[{"given":"Rui","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, RenJi Hospital, School of Medicine Shanghai Jiao Tong University Shanghai China 200127"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiliang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, RenJi Hospital, School of Medicine Shanghai Jiao Tong University Shanghai China 200127"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhujia","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, RenJi Hospital, School of Medicine Shanghai Jiao Tong University Shanghai China 200127"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, RenJi Hospital, School of Medicine Shanghai Jiao Tong University Shanghai China 200127"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingang","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, RenJi Hospital, School of Medicine Shanghai Jiao Tong University Shanghai China 200127"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, RenJi Hospital, School of Medicine Shanghai Jiao Tong University Shanghai China 200127"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Bai","sequence":"additional","affiliation":[{"name":"Shanghai Center for Systems Biomedicine Shanghai Jiao Tong University Shanghai China 200240"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Institute of Immunology, School of Medicine Shanghai Jiao Tong University Shanghai China 200025"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihong","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, RenJi Hospital, School of Medicine Shanghai Jiao Tong University Shanghai China 200127"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,4,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/wound.2012.0363"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-006-0651-7"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00106-010-2124-8"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(99)00250-6"},{"key":"e_1_2_7_6_1","first-page":"475","article-title":"Effects of antisense oligonucleotides on the expression of focal adhesion kinase gene and collagen synthesis in the cultured human fibroblasts of hypertrophic scar","volume":"24","author":"Chen R","year":"2008","journal-title":"Zhonghua zheng xing wai ke za zhi"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.21725"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)61252-7"},{"key":"e_1_2_7_9_1","doi-asserted-by":"crossref","first-page":"2375","DOI":"10.1242\/jcs.113.13.2375","article-title":"Contraction of collagen matrices mediated by alpha2beta1A and alpha (v) beta3 integrins","volume":"113","author":"Cooke ME","year":"2000","journal-title":"J Cell Sci"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700975"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/35078107"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12371710"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/418244a"},{"key":"e_1_2_7_14_1","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0891-8422(23)01171-0","article-title":"Hypertrophic scars and keloids","volume":"18","author":"Haverstock BD","year":"2001","journal-title":"Clin Podiatr Med Surg"},{"key":"e_1_2_7_15_1","first-page":"011","article-title":"Experimental study of mechanism of focal adhesion kinase in hypertrophic scar [J]","volume":"5","author":"He Z\u2010Y","year":"2005","journal-title":"Acta Universitatis Medicinalis Secondae Shanghai"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11926-009-0017-1"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E02-11-0729"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2011.09.011"},{"key":"e_1_2_7_19_1","volume-title":"Tumors of the soft tissues","author":"Kempson RL","year":"1998"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40259-012-0003-4"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.03-1273rev"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E06-12-1121"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.2174\/1566523044578004"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/BCR.0B013E318031A151"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008208209160259"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-0361"},{"key":"e_1_2_7_27_1","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/S0749-0712(21)00204-3","article-title":"Control of wound contraction. Basic and clinical features","volume":"16","author":"Nedelec B","year":"2000","journal-title":"Hand clinics"},{"key":"e_1_2_7_28_1","first-page":"523","article-title":"Pathological scarring: strategic interventions","volume":"168","author":"Oleary R","year":"2002","journal-title":"Eur J Surg"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00262.2003"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-4725.2011.02201.x"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.1999.5580"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-4310(97)80541-2"},{"key":"e_1_2_7_33_1","first-page":"757","article-title":"What treatment is best for hypertrophic scars and keloids","volume":"60","author":"Williams C","year":"2011","journal-title":"J Fam Pract"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2574"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2277"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12606979"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbin.10265","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.10265","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,24]],"date-time":"2024-05-24T06:59:39Z","timestamp":1716533979000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.10265"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4,24]]},"references-count":35,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2014,7]]}},"alternative-id":["10.1002\/cbin.10265"],"URL":"https:\/\/doi.org\/10.1002\/cbin.10265","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,4,24]]},"assertion":[{"value":"2013-09-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-01-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-04-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}