{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T20:18:06Z","timestamp":1776802686644,"version":"3.51.2"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,2,2]],"date-time":"2015-02-02T00:00:00Z","timestamp":1422835200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81171709"],"award-info":[{"award-number":["81171709"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>In the past decades increasing lines of evidence have demonstrated that adipose tissue, as an endocrine organ plays a central role in metabolic homeostasis and its related maladies. CCAAT\/enhancer\u2010binding protein (C\/EBP) family members and the nuclear receptor peroxisome proliferator\u2010activated receptor gamma (PPAR\u03b3) were known to be the vital transcription factors in the regulation of adipogenesis. However, the exact mechanism for increased marrow fat in patients with bone metabolic diseases, such as osteoporosis, is still poorly understood. Herein, we studied the expression pattern of PPAR\u03b3 and C\/EBPs in human bone marrow mesenchymal stem cell (hBMSC) adipogenesis and evaluated the effects of individual components of an adipogenic cocktail on the differentiation and transcription factor expression. We furthermore examined whether the ERK signaling pathway was involved in mediating these effects. These findings showed that C\/EBP\u03b2 and C\/EBP\u03b4 were detected in undifferentiated hBMSC and maintained during the whole process of adipogenesis, and could initiate the expression of PPAR\u03b31 under the treatment of dexamethasone and IBMX. Subsequently, the activation of PPAR\u03b31 by indomethacin, its exogenous ligand, activated C\/EBP\u03b1, which, together with IBMX, up\u2010regulated PPAR\u03b32 expression and therefore the fullest adipogenesis. Insulin and its downstream signal pathway extracellular signal\u2010regulated kinases (ERK), however, were found not necessary for hBMSC adipogenesis. Our results revealed some unique characteristics of human adipocyte formation, which may help to understand the molecular mechanisms of bone marrow adipogenesis and give insights into the treatment of osteoporosis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10415","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T09:05:05Z","timestamp":1418979905000},"page":"457-465","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Expression patterns of transcription factor PPAR\u03b3 and C\/EBP family members during in vitro adipogenesis of human bone marrow mesenchymal stem cells"],"prefix":"10.1002","volume":"39","author":[{"given":"Xiao\u2010Yi","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Orthopedics First Affiliated Hospital of Sun Yat\u2010sen University Guangzhou 510080 Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Yong","family":"Chen","sequence":"additional","affiliation":[{"name":"Center for Stem Cell Biology and Tissue Engineering The Key Laboratory for Stem Cells and Tissue Engineering Ministry of Education Sun Yat\u2010Sen University Guangzhou 510080 Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zi\u2010Ji","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics First Affiliated Hospital of Sun Yat\u2010sen University Guangzhou 510080 Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics First Affiliated Hospital of Sun Yat\u2010sen University Guangzhou 510080 Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Ming","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Orthopedics First Affiliated Hospital of Sun Yat\u2010sen University Guangzhou 510080 Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei\u2010Hua","family":"Yu","sequence":"additional","affiliation":[{"name":"Center for Stem Cell Biology and Tissue Engineering The Key Laboratory for Stem Cells and Tissue Engineering Ministry of Education Sun Yat\u2010Sen University Guangzhou 510080 Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andy Peng","family":"Xiang","sequence":"additional","affiliation":[{"name":"Center for Stem Cell Biology and Tissue Engineering The Key Laboratory for Stem Cells and Tissue Engineering Ministry of Education Sun Yat\u2010Sen University Guangzhou 510080 Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,2,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.5.9.1538"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3198"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2005.04.029"},{"issue":"36","key":"e_1_2_8_5_1","doi-asserted-by":"crossref","first-page":"27815","DOI":"10.1074\/jbc.M003593200","article-title":"Modulation of the murine peroxisome proliferator\u2010activated receptor gamma 2 promoter activity by CCAAT\/enhancer\u2010binding proteins","volume":"275","author":"Elberg G","year":"2000","journal-title":"J Biol Chem"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2006.07.001"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M605077200"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100797200"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2009.02.002"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/oby.2003.11"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034114"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.6.3406"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20617"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.28.2040"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E08-04-0420"},{"issue":"5411","key":"e_1_2_8_16_1","doi-asserted-by":"crossref","first-page":"p. 143","DOI":"10.1126\/science.284.5411.143","article-title":"Multilineage potential of adult human mesenchymal stem cells","volume":"284","author":"Pittenger MF","year":"1999","journal-title":"Science"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-10-47"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.948702"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2003.10.010"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1677\/jme.1.01918"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0503891102"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0137044100"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.77.061307.091829"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0607378104"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2011.11.013"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0703407104"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbin.10415","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.10415","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T01:29:42Z","timestamp":1696642182000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.10415"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2,2]]},"references-count":25,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,4]]}},"alternative-id":["10.1002\/cbin.10415"],"URL":"https:\/\/doi.org\/10.1002\/cbin.10415","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2,2]]},"assertion":[{"value":"2014-07-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-12-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-02-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}