{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T23:38:41Z","timestamp":1783813121003,"version":"3.55.0"},"reference-count":26,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,5,7]],"date-time":"2015-05-07T00:00:00Z","timestamp":1430956800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2015,5,7]],"date-time":"2015-05-07T00:00:00Z","timestamp":1430956800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2012J01209"],"award-info":[{"award-number":["2012J01209"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Opening Research Foundation of Key Laboratory of Biomedical Material in Tianjin city"},{"name":"Scientific Research Foundation in Fuzhou University","award":["2013-XQ-24"],"award-info":[{"award-number":["2013-XQ-24"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Osteoarthritis, a common joint degeneration, can cause breakdown of articular cartilage with the presence of lubricin metabolic abnormalities. Lubricin is a multi\u2010level chondroprotective mucinous glycoprotein in articular joints. Joint defect and infection is elevated and accompanied by accelerated cartilage lesions involving degradation and loss of lubricin. However, a novel, heterocyclic compound called kartogenin (KGN) was discovered to stimulate chondrogenic differentiation of bone\u2010derived mesenchymal stem cells (BMSCs). And the synergistic effect of transforming growth factor\u2010\u03b21 (TGF\u2010\u03b21) and bone morphogenetic protein\u20107 (BMP\u20107) could provoke lubricin accumulation. This paper attempted to explore the connection between accumulation of lubricin and the effect of TGF\u2010\u03b21, BMP\u20107 and\/or KGN. Hence, we investigated the expression and secretion of lubricin in BMSCs treated with different combinations of TGF\u2010\u03b21, BMP\u20107, and\/or KGN. Using an in vitro BMSCs system, we observed the content of lubricin from BMSCs treated with TGF\u2010\u03b21, BMP\u20107, and KGN was the highest at both the protein level and the gene level. The accumulation of lubricin was enhanced coordinately by the increase of synthesis and decrease of degradation possibly via\n                      <jats:italic>c\u2010Myc<\/jats:italic>\n                      and\n                      <jats:italic>adamts5<\/jats:italic>\n                      pathway. These results further suggested that supplementation of the defect parts with lubricin by using growth factors and small molecules showed a promising potential on preventing joint deterioration in patients with acquired or genetic deficiency of lubricin in the future of regenerative medicine.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cbin.10476","type":"journal-article","created":{"date-parts":[[2015,4,10]],"date-time":"2015-04-10T00:16:52Z","timestamp":1428625012000},"page":"1026-1035","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Kartogenin, transforming growth factor\u2010\u03b21 and bone morphogenetic protein\u20107 coordinately enhance lubricin accumulation in bone\u2010derived mesenchymal stem cells"],"prefix":"10.1002","volume":"39","author":[{"given":"Chun","family":"Liu","sequence":"first","affiliation":[{"name":"Institute of Biomedical and Pharmaceutical Technology Fuzhou University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueqin","family":"Ma","sequence":"additional","affiliation":[{"name":"Institute of Biomedical and Pharmaceutical Technology Fuzhou University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Biomedical and Pharmaceutical Technology Fuzhou University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,5,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar3793"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-010-9949-9"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwg082"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2013.123"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cocis.2010.07.002"},{"key":"e_1_2_8_7_1","volume-title":"In Vitro Effects of BMP\u20102, BMP\u20107, AND BMP\u201013 on Differentiated and Undifferentiated Mouse Mesenchymal Stem Cells","author":"Dorman LJ","year":"2012"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2014.09.011"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.2217\/rme.12.41"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.534"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2013.0047"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1215157"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v013a04"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-007-9576-2"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2013.05.069"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008209609028881"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.21215"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcibr1204283"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00167-012-1920-y"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.4081\/ejh.2011.e31"},{"key":"e_1_2_8_21_1","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1160\/VCOT-07-02-0018","article-title":"The role of bone morphogenetic proteins in articular cartilage development, homeostasis and repair","volume":"20","author":"Oshin AO","year":"2007","journal-title":"Vet Comp Orthop Traumatol"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3005409"},{"key":"e_1_2_8_23_1","first-page":"8069","article-title":"The Molecular Basis of Articular Lubrication. 1. Purification and properties of a lubricating fraction from bovine synovial fluid","volume":"247","author":"Swann DA","year":"1972","journal-title":"J Biol Chem"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1219289110"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20521"},{"key":"e_1_2_8_26_1","first-page":"2","article-title":"Kartogenin induces cartilage\u2010like tissue formation in tendon\u2010bone junction","author":"Zhang J","year":"2014","journal-title":"Bone Res"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3143"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbin.10476","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.10476","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cbin.10476","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.10476","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T21:53:02Z","timestamp":1769723582000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.10476"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,7]]},"references-count":26,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2015,9]]}},"alternative-id":["10.1002\/cbin.10476"],"URL":"https:\/\/doi.org\/10.1002\/cbin.10476","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,7]]},"assertion":[{"value":"2014-12-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-03-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-05-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}