{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T05:39:00Z","timestamp":1776231540729,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,9,7]],"date-time":"2015-09-07T00:00:00Z","timestamp":1441584000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004515","name":"Universiti Kebangsaan Malaysia","doi-asserted-by":"publisher","award":["UKM-DLP-2012-008"],"award-info":[{"award-number":["UKM-DLP-2012-008"]}],"id":[{"id":"10.13039\/501100004515","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>The alarming rate of increase in myocardial infarction and marginal success in efforts to regenerate the damaged myocardium through conventional treatments creates an exceptional avenue for cell\u2010based therapy. Adult bone marrow mesenchymal stem cells (MSCs) can be differentiated into cardiomyocytes, by treatment with 5\u2010azacytidine, thus, have been anticipated as a therapeutic tool for myocardial infarction treatment. In this study, we investigated the ability of basic fibroblastic growth factor (bFGF) and hydrocortisone as a combined treatment to stimulate the differentiation of MSCs into cardiomyocytes. MSCs were isolated from sternal marrow of patients undergoing heart surgery (CABG). The isolated cells were initially monitored for the growth pattern, followed by characterization using ISCT recommendations. Cells were then differentiated using a combination of bFGF and hydrocortisone and evaluated for the expression of characteristic cardiac markers such as CTnI, CTnC, and Cnx43 at protein level using immunocytochemistry and flow cytometry, and CTnC and CTnT at mRNA level. The expression levels and pattern of the cardiac markers upon analysis with ICC and qRT\u2010PCR were similar to that of 5\u2010azacytidine induced cells and cultured primary human cardiomyocytes. However, flow cytometric evaluation revealed that induction with bFGF and hydrocortisone drives MSC differentiation to cardiomyocytes with a marginally higher efficiency. These results indicate that combination treatment of bFGF and hydrocortisone can be used as an alternative induction method for cardiomyogenic differentiation of MSCs for future clinical applications.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10536","type":"journal-article","created":{"date-parts":[[2015,8,20]],"date-time":"2015-08-20T06:41:41Z","timestamp":1440052901000},"page":"55-64","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Cardiomyogenic differentiation of human sternal bone marrow mesenchymal stem cells using a combination of basic fibroblast growth factor and hydrocortisone"],"prefix":"10.1002","volume":"40","author":[{"given":"Pezhman","family":"Hafez","sequence":"first","affiliation":[{"name":"Tissue Engineering Centre Universiti Kebangsaan Malaysia Medical Center Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinsmon","family":"Jose","sequence":"additional","affiliation":[{"name":"Tissue Engineering Centre Universiti Kebangsaan Malaysia Medical Center Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiplu R.","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Tissue Engineering Centre Universiti Kebangsaan Malaysia Medical Center Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min Hwei","family":"Ng","sequence":"additional","affiliation":[{"name":"Tissue Engineering Centre Universiti Kebangsaan Malaysia Medical Center Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. H. I.","family":"Ruszymah","sequence":"additional","affiliation":[{"name":"Tissue Engineering Centre Universiti Kebangsaan Malaysia Medical Center Kuala Lumpur Malaysia"},{"name":"Department of Physiology Faculty of Medicine Universiti Kebangsaan Malaysia Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramzisham","family":"Abdul Rahman Mohd","sequence":"additional","affiliation":[{"name":"Division of Cardiothoracic Surgery Department of Surgery Universiti Kebangsaan Malaysia Medical Centre Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,9,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2009.02.093"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOT.0000000000000043"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1510\/icvts.2007.157875"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-989328"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2011.10.002"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.4236\/scd.2013.31003"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-472X(00)00160-0"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/5.12.1583"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3835(87)90145-5"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.113.006591"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405957102"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00534.2001"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/14653240600855905"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2003.10.006"},{"key":"e_1_2_9_16_1","doi-asserted-by":"crossref","unstructured":"FrancoD Ar\u00e1negaA Lozano\u2010VelascoE(2011) Transcriptional networks of embryonic stem cell\u2010derived cardiomyogenesis: INTECH open access publisher.","DOI":"10.5772\/24459"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-0061"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00369"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2007.11.040"},{"key":"e_1_2_9_20_1","first-page":"1217","article-title":"[Activin A and BMP\u20104 induce cardiomyocyte\u2010like cells differentiation of human amniotic epithelial cells]","volume":"28","author":"Han X","year":"2011","journal-title":"Sheng wu yi xue gong cheng xue za zhi= Journal of biomedical engineering= Shengwu yixue gongchengxue zazhi"},{"key":"e_1_2_9_21_1","first-page":"1037","article-title":"Cell cycle homogeneity in bone marrow samples from different sites: flow cytometric evaluation of multiple samples from sheep","volume":"11","author":"Harker G","year":"1983","journal-title":"Exp Hematol"},{"issue":"8","key":"e_1_2_9_22_1","first-page":"156","article-title":"Human mesenchymal stem cells differentiation to skeletal muscle cells in vitro study","volume":"3","author":"HassanHeidari M","year":"2013","journal-title":"J Basic Appl Sci Res"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(91)90192-Q"},{"key":"e_1_2_9_24_1","first-page":"242","article-title":"Human sternal mesenchymal stem cells: isolation, characterization and cardiomyogenic differentiation","volume":"26","author":"Hsu CP","year":"2010","journal-title":"Acta Cardiologica Sinica"},{"key":"e_1_2_9_25_1","first-page":"181","article-title":"Effect of basic fibroblast growth factor on cardiomyocyte differentiation from mouse embryonic stem cells","volume":"28","author":"Khezri S","year":"2007","journal-title":"Saudi Med J"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2009.10.001"},{"key":"e_1_2_9_27_1","first-page":"876","article-title":"Autologous transplantation of bone marrow mononuclear cells improved heart function after myocardial infarction","volume":"25","author":"Lin GS","year":"2004","journal-title":"Acta Pharmacol Sin"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(03)00265-7"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0056554"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI5298"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.227512"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/35070587"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100090504"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2010.09.001"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI23418"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(03)00286-4"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1995.1102"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002020203"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01908162"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1161\/hc0102.101442"},{"key":"e_1_2_9_41_1","doi-asserted-by":"crossref","unstructured":"VindigniV GiatsidisG RehoF Dalla VeneziaE MammanaM FrancoB(2013) Adipose derived stem cells: current state of the art and prospective role in regenerative medicine and tissue engineering. Regenerative medicine and tissue engineering ISBN 953\u201078.","DOI":"10.5772\/55924"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.880181212"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1042\/BC20040099"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e02-02-0105"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbin.10536","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.10536","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T04:06:47Z","timestamp":1696565207000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.10536"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9,7]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,1]]}},"alternative-id":["10.1002\/cbin.10536"],"URL":"https:\/\/doi.org\/10.1002\/cbin.10536","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9,7]]},"assertion":[{"value":"2015-06-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-08-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-09-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}