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However, SCNT animals exhibit DNA methylation and genomic imprinting abnormalities. These abnormalities may be due to the faulty epigenetic reprogramming of donor cells. To investigate the consequence of SCNT on the genomic imprinting and global methylation in the donor cells, growth patterns and apoptosis of cloned goat fibroblast cells (CGFCs) at passage 7 were determined. Growth patterns in CGFCs were similar to the controls; however, the growth rate in log phase was lower and apoptosis in CGFCs were significantly higher (<jats:italic>P\u2009<\/jats:italic>&lt;\u20090.01). In addition, quantitative expression analysis of three DNA methyltransferases (Dnmt) and two imprinted genes (<jats:italic>H19, IGF2R<\/jats:italic>) was conducted in CGFCs: <jats:italic>Dnmt1<\/jats:italic> and <jats:italic>Dnmt3b<\/jats:italic> expression was significantly reduced (<jats:italic>P\u2009<\/jats:italic>&lt;\u20090.01), and <jats:italic>H19<\/jats:italic> expression was decreased sixfold (<jats:italic>P\u2009<\/jats:italic>&lt;\u20090.01); however, the expression of <jats:italic>Dnmt3a<\/jats:italic> was unaltered and <jats:italic>IGF2R<\/jats:italic> expression was significantly increased (<jats:italic>P\u2009<\/jats:italic>&lt;\u20090.05). Finally, we used bisulfite sequencing PCR to compare the DNA methylation patterns in differentially methylated regions (DMRs) of <jats:italic>H19<\/jats:italic> and <jats:italic>IGF2R<\/jats:italic>. The DMRs of <jats:italic>H19<\/jats:italic> (<jats:italic>P\u2009<\/jats:italic>&lt;\u20090.01) and <jats:italic>IGF2R<\/jats:italic> (<jats:italic>P\u2009<\/jats:italic>&lt;\u20090.01) were both highly methylated in CGFCs. These results indicate that the global genome might be hypomethylated. Moreover, there is an aberrant expression of imprinted genes and DMR methylation in CGFCs.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10540","type":"journal-article","created":{"date-parts":[[2015,8,28]],"date-time":"2015-08-28T04:24:59Z","timestamp":1440735899000},"page":"74-82","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Abnormal expression of DNA methyltransferases and genomic imprinting in cloned goat fibroblasts"],"prefix":"10.1002","volume":"40","author":[{"given":"Yongjie","family":"Wan","sequence":"first","affiliation":[{"name":"Jiangsu Livestock Embryo Engineering Laboratory College of Animal Science and Technology Nanjing Agricultural University Nanjing   210095 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingtian","family":"Deng","sequence":"additional","affiliation":[{"name":"Jiangsu Livestock Embryo Engineering Laboratory College of Animal Science and Technology Nanjing 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