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A pcDNA3.1 expression vector of HBx gene was built and transfected into JEG\u20103 and HTR\u20108 human placental trophoblastic cell lines, respectively. After transfection for 48\u2009h, RT\u2010PCR and immunofluorescence analyses showed that HBx mRNA and protein expression was detected in JEG\u20103 and HTR\u20108 cells. Flow cytometry revealed that early apoptosis of JEG\u20103 and HTR\u20108 cells was reduced by pcDNA\u2010HBx transfection. Immunofluorescence and Western blotting showed that PI3K and p\u2010Akt were significantly upregulated in HTR\u20108 cells. HBx ectopic expression did not change the viability of JEG\u20103 and HTR\u20108 cells when the PI3K\/Akt pathway was blocked by its specific inhibitor LY294002. Moreover, the pcDNA\u2010PI3K expression vector and pcDNA\u2010HBx were transfected individually or co\u2010transfected into the cells. The results showed that pcDNA\u2010PI3K\/pcDNA\u2010HBx co\u2010transfection promoted the expression of PI3K protein compared with the pcDNA\u2010PI3K transfection group but did not increase the expression of HBx protein compared with pcDNA\u2010HBx transfection group. In conclusion, HBx gene can be transferred into JEG\u20103 and HTR\u20108 human placental trophoblastic cell lines and cause inhibition of cell apoptosis. Its effect of apoptosis inhibition is related to the activation of the PI3K\/Akt signaling pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cbin.10608","type":"journal-article","created":{"date-parts":[[2016,3,29]],"date-time":"2016-03-29T21:42:23Z","timestamp":1459287743000},"page":"708-715","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["HBxAg suppresses apoptosis of human placental trophoblastic cell lines via activation of the PI3K\/Akt pathway"],"prefix":"10.1002","volume":"40","author":[{"given":"Weimin","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Gynecology and Obstetrics the First Affiliated Hospital Xi'an Jiaotong University Xi'an City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya'e","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics People's Hospital of Tongchuan (North 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