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However, expression and possible role of ZO\u20101 in human dental pulp cells (hDPCs) during repair process remains unknown. Here, we investigated the expression of ZO\u20101 in hDPCs and the relationship with odontoblast differentiation. We found the processes of two adjacent cells were fused and formed junction\u2010like structure using scanning electron microscopy. Fluorescence immunoassay and Western blot confirmed ZO\u20101 expression in hDPCs. Especially, ZO\u20101 was high expressed at the cell\u2013cell junction sites. More interestingly, ZO\u20101 accumulated at the leading edge of lamellipodia in migrating cells when a scratch assay was performed. Furthermore, ZO\u20101 gradual increased during odontoblast differentiation and ZO\u20101 silencing greatly inhibited the differentiation. ZO\u20101 binds directly to actin filaments and RhoA\/ROCK signaling mainly regulates cell cytoskeleton, thus RhoA\/ROCK might play a role in regulating ZO\u20101. Lysophosphatidic acid (LPA) and Y\u201027632 were used to activate and inhibit RhoA\/ROCK signaling, respectively, with or without mineralizing medium. In normal cultured hDPCs, RhoA activation increased ZO\u20101 expression and especially in intercellular contacts, whereas ROCK inhibition attenuated the effects induced by LPA. However, expression of ZO\u20101 was upregulated by Y\u201027632 but not significantly affected by LPA after odontoblast differentiation. Hence, ZO\u20101 highly expresses in cell\u2013cell junctions and is related to odontoblast differentiation, which may contribute to dental pulp repair or even the formation of an odontoblast layer. RhoA\/ROCK signaling is involved in the regulation of ZO\u20101.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10617","type":"journal-article","created":{"date-parts":[[2016,4,25]],"date-time":"2016-04-25T06:06:40Z","timestamp":1461564400000},"page":"787-795","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Novel role of zonula occludens\u20101: A tight junction protein closely associated with the odontoblast differentiation of human dental pulp cells"],"prefix":"10.1002","volume":"40","author":[{"given":"Jue","family":"Xu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases, Department of Preventive Dentistry West China Hospital of Stomatology, Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meiying","family":"Shao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases, College of Life Sciences Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongying","family":"Pan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases, Department of Operative Dentistry and Endodontics West China Hospital of Stomatology, Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huning","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases, Department of Operative Dentistry and Endodontics West China Hospital of Stomatology, Sichuan University Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Cheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases, Department of Preventive Dentistry West China Hospital of Stomatology, Sichuan University Chengdu 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