{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T06:07:59Z","timestamp":1777529279165,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,6,28]],"date-time":"2016-06-28T00:00:00Z","timestamp":1467072000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Placental trophoblast differentiation involves the continuous fusion of mononuclear cytotrophoblasts. However, except for syncytin, little is known about the detailed mechanisms underlying trophoblast fusion. A previous study indicated that lipid rafts play an important role in HTLV\u20101 syncytium formation. To identify proteins that may be involved in placental trophoblast differentiation, we examined stomatin, an important lipid\u2010raft protein that localizes to detergent\u2010resistant membrane domains. The syncytium and human chorionic gonadotropin (\u03b2\u2010hCG; a marker of placental trophoblast differentiation) were visualized by immunofluorescence staining. We found that overexpression of stomatin in the nonfusogenic JEG\u20103 cell line caused syncytium formation and increased the fusion index of cells. Treating these cells with N<jats:sup>6<\/jats:sup>,2\u2032\u2010O\u2010dibutyryladenosine 3\u2032,5\u2032\u2010cyclic monophosphate further increased cell fusion by stomatin. \u03b2\u2010hCG was found in a few JEG\u20103 cells overexpressing stomatin at 48\u2009h, and its levels increased dramatically at 72\u2009h along with the formation of the multinuclear syncytium. RNA interference was used to decrease stomatin expression in BeWo cells, a fusogenic human choriocarcinoma cell line. After knockdown for 72\u2009h, stomatin levels decreased by almost 95%. The fusion indexes of control and stomatin\u2010knockdown cells at 72\u2009h were 9.4 and 6.5%, respectively. Our data indicated that stomatin could trigger syncytium formation and upregulate \u03b2\u2010hCG for cell fusion in nonfusogenic JEG\u20103 cells. Downregulation of stomatin slightly inhibited the fusion index of fusogenic BeWo cells. Thus, these data suggested that stomatin plays an important role in trophoblast differentiation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10636","type":"journal-article","created":{"date-parts":[[2016,6,16]],"date-time":"2016-06-16T07:32:04Z","timestamp":1466062324000},"page":"926-933","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Over\u2010expression of stomatin causes syncytium formation in nonfusogenic JEG\u20103 choriocarcinoma placental cells"],"prefix":"10.1002","volume":"40","author":[{"given":"Tung\u2010Wei","family":"Chen","sequence":"first","affiliation":[{"name":"Institute of Biophotonics National Yang\u2010Ming University No. 155, Sec. 2, Linong Street, Taipei Taiwan 112, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong\u2010Wen","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology National Yang\u2010Ming University No. 155, Sec. 2, Linong Street, Taipei Taiwan 112, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Jia","family":"Liou","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology National Yang\u2010Ming University No. 155, Sec. 2, Linong Street, Taipei Taiwan 112, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jui\u2010Hao","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology National Yang\u2010Ming University No. 155, Sec. 2, Linong Street, Taipei Taiwan 112, ROC"},{"name":"Institute of Biochemistry National Yang\u2010Ming University No. 155, Sec. 2, Linong Street, Taipei Taiwan 112, ROC"},{"name":"Taiwan International Graduate Program in Molecular Medicine National Yang\u2010Ming University and Academia Sinica No. 155, Sec. 2, Linong Street, Taipei Taiwan 112, 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