{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T02:16:54Z","timestamp":1789611414143,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,6,28]],"date-time":"2016-06-28T00:00:00Z","timestamp":1467072000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>The remodeling of cardiac gap junctions contributes to various arrhythmias in a diabetic heart. We previously reported that Epigallocatechin\u20103\u2010gallate (EGCG) attenuated connexin43 (Cx43) protein downregulation induced by high glucose (HG) in neonatal rat cardiomyocytes, but Cx43 mRNA expression was not affected. It indicated the possible mechanisms of post\u2010transcriptional regulation, which still remains unclear. As microRNAs (miRNAs) regulate gene expression widely at post\u2010transcriptional level, we measured miR\u20101\/206 in cardiomyocytes treated with HG and EGCG by quantitative RT\u2010PCR and investigated their relationship with signal transduction pathways. The results showed that HG induced miR\u20101\/206 elevation by PKC MAPK pathway. Moreover, we tested the negative regulation effect of miR\u20101\/206 on Cx43 protein by miRNAs transfection. EGCG, however, nearly abolished the HG\u2010induced miR\u20101 augmentation via P38 MAPK pathway. Therefore, our study suggested that PKC\u2010activated miR\u20101\/206 expression might contribute to Cx43 downregulation in HG\u2010treated cardiomyocytes, and EGCG conferred protective effect by inhibiting miR\u20101 elevation via P38 MAPK pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cbin.10637","type":"journal-article","created":{"date-parts":[[2016,6,16]],"date-time":"2016-06-16T03:32:05Z","timestamp":1466047925000},"page":"934-942","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["P38 MAPK\/miR\u20101 are involved in the protective effect of EGCG in high glucose\u2010induced Cx43 downregulation in neonatal rat cardiomyocytes"],"prefix":"10.1002","volume":"40","author":[{"given":"Lu","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Medicine Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University 3 East Qingchun Road Hangzhou Zhejiang 310016 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