{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T23:07:06Z","timestamp":1777417626300,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2016,7,12]],"date-time":"2016-07-12T00:00:00Z","timestamp":1468281600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81200795"],"award-info":[{"award-number":["81200795"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Mechanical strain plays an important role in bone formation and resorption during orthodontic tooth movement. The mechanism has not been fully studied, and the process becomes complex with increased amounts of periodontal patients seeking orthodontic care. Our aims were to elucidate the combined effects of proinflammatory cytokines and intermittent cyclic strain (ICS) on the osteogenic capacity of human periodontal ligament cells. Cultured human periodontal ligament cells were exposed to proinflammatory cytokines (interleukin\u20101\u03b2 5\u2009ng\/mL and tumor necrosis factor\u2010\u03b1 10\u2009ng\/mL) for 1 and 5 days, and ICS (0.5\u2009Hz, 12% elongation) was applied for 4\u2009h per day. The autocrine of inflammatory cytokines was measured by enzyme\u2010linked immunosorbent assay. The expression of osteoblast markers runt\u2010related transcription factor 2 and rabbit collagen type I was determined using real\u2010time polymerase chain reaction and Western blot. The osteogenic capacity was also detected by alkaline phosphatase (ALP) staining, ALP activity, and alizarin red staining. We demonstrated that ICS impaired the osteogenic capacity of human periodontal ligament cells when incubated with proinflammatory cytokines, as evidenced by the low expression of ALP staining, low ALP activity, reduced alizarin red staining, and reduced osteoblast markers. These data, for the first time, suggest that ICS has a negative effect on the inductive inhibition of osteogenicity in human PDL cells mediated by proinflammatory cytokines.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10641","type":"journal-article","created":{"date-parts":[[2016,6,30]],"date-time":"2016-06-30T07:25:31Z","timestamp":1467271531000},"page":"999-1007","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Combined effects of proinflammatory cytokines and intermittent cyclic mechanical strain in inhibiting osteogenicity in human periodontal ligament cells"],"prefix":"10.1002","volume":"40","author":[{"given":"Chaofan","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Orthodontics School of Stomatology Wenzhou Medical University Wenzhou 325027 Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijiao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthodontics School of Stomatology Wenzhou Medical University Wenzhou 325027 Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinlian","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Periodontics School of Stomatology Wenzhou Medical University Wenzhou 325027 Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhensheng","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Orthodontics School of Stomatology Wenzhou Medical University Wenzhou 325027 Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bibo","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Orthodontics School of Stomatology Wenzhou Medical University Wenzhou 325027 Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenbin","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Orthodontics School of Stomatology Wenzhou Medical University Wenzhou 325027 Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manman","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Periodontics School of Stomatology Wenzhou Medical University Wenzhou 325027 Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rongdang","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Orthodontics School of Stomatology Wenzhou Medical University Wenzhou 325027 Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Periodontics School of Stomatology Wenzhou Medical University Wenzhou 325027 Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,7,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2010.11.010"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.2006.00400.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2013.14"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2009.01.013"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2000.71.10.1535"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.3.391"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.job.2013.09.001"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kei282"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1067\/mod.2003.99"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2013.07.027"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2013.2070"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612033453659"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.440"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345010800050601"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2003.08.003"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2010.08.004"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00056-010-1031-7"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejo\/cjp053"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67728-8"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2005.06.006"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16627-0"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2013.05.015"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910708601214"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345960750030501"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.05.025"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12170"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbin.10641","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.10641","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T22:30:01Z","timestamp":1696631401000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.10641"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7,12]]},"references-count":26,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2016,9]]}},"alternative-id":["10.1002\/cbin.10641"],"URL":"https:\/\/doi.org\/10.1002\/cbin.10641","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7,12]]},"assertion":[{"value":"2016-03-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-06-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-07-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}