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We have earlier standardized a three\u2010step differentiation protocol to generate functional hepatocyte\u2010like cells (HLCs) from hESCs, which expressed the major hepatic markers. We have also found that the HLCs remain stable and functional even after extended period of in vitro culture and cryopreservation. In the present study, we have aimed to investigate the therapeutic potential of cryopreserved\u2010thawed hESC\u2010derived hepatic progenitor cells following transplantation in carbon tetrachloride\u2010induced fibrotic rat livers. Significant therapeutic effects, including improved hepatic histology and normal serum biochemistry of hepatic enzymes along with increased survival rate, were observed in the cell transplanted rats. This result is an encouraging indication to develop methods for clinical application of hESC\u2010derived hepatic lineage cells.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10649","type":"journal-article","created":{"date-parts":[[2016,7,25]],"date-time":"2016-07-25T09:55:24Z","timestamp":1469440524000},"page":"1107-1115","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cryopreserved hepatic progenitor cells derived from human embryonic stem cells can arrest progression of liver fibrosis in rats"],"prefix":"10.1002","volume":"40","author":[{"given":"Arundhati","family":"Mandal","sequence":"first","affiliation":[{"name":"Regenerative Medicine, Reliance Life Sciences Pvt. Ltd. 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