{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T09:14:41Z","timestamp":1696670081366},"reference-count":18,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,8,9]],"date-time":"2016-08-09T00:00:00Z","timestamp":1470700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label \/><jats:p>A study was conducted to examine the effect of phenamil on adipogenic differentiation and expression of key adipogenic transcripts in hen preadipocytes. Preadipocytes were isolated from 20\u2010week old Single Comb White Leghorn hens (<jats:italic>Gallas gallus<\/jats:italic>, Lohman strain). The experiment lasted for 48\u2009h and had six treatments. Non\u2010treated control (C) cells, cells treated with dexamethasone, 3\u2010isobutyl\u20101\u2010methylxanthine, insulin, and oleic acid (DMIOA) (T1), DMIOA\u2009+\u200915\u2009\u03bcM phenamil (T2), DMIOA\u2009+\u200930\u2009\u03bcM phenamil (T3), 15\u2009\u03bcM phenamil alone (T4), and 30\u2009\u03bcM phenamil alone (T5). Neutral lipid accumulation and the mRNA expression of key adipogenic transcripts were measured in all treatments and compared. Lipid accumulation was detected in T1, T2, and T3 only. Expression of peroxisome proliferator receptor\u2010activator gamma 2 (PPAR\u03b32), the core enhancer binding protein \u03b1 (C\/EBP\u03b1), C\/EBP\u03b2, fatty acid binding protein 4 (FABP4), and lipoprotein lipase (LPL) as well as ETS variant 4 (ETV4) and 5 was higher (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) in T2, T3, T4, and T5 compared to C. Expression of these transcripts was higher (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) in T2 and T3 compared to T4 and T5. The core enhancer binding protein \u03b1, C\/EBP\u03b2, and FABP4 were highly expressed (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) in T1 compared to C. However, the expression of PPAR\u03b32, LPL, and ETV4 and ETV5 was not significantly different. Expression of C\/EBP\u03b1, C\/EBP\u03b2, and FABP4 was higher (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) in T2 and T3 compared to T1. Expression of sterol regulatory element binding protein 1 (SREBP1) and leptin receptor (LEPR) was not significantly different among the treatments. In conclusion, phenamil enhances DMIOA\u2010induced adipogenic differentiation of hen preadipocytes but does not induce adipogenesis by itself.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10651","type":"journal-article","created":{"date-parts":[[2016,7,27]],"date-time":"2016-07-27T09:16:54Z","timestamp":1469611014000},"page":"1123-1128","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phenamil enhances the adipogenic differentiation of hen preadipocytes"],"prefix":"10.1002","volume":"40","author":[{"given":"Alemu","family":"Regassa","sequence":"first","affiliation":[{"name":"Department of Animal Science University of Manitoba Winnipeg Manitoba Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kye Won","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Food Science and Biotechnology Sungkyunkwan University Suwon 16419 Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Woo Kyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Poultry Science University of Georgia Athens Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,8,9]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"Guide to the care and use of experimental animals","year":"2009"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2006.07.001"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100797200"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.8.4333"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2008.11.005"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2008.07.002"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2005.04.013"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M008490"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00002-09"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-1231-z"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.10122"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.948702"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.14.11.1293"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2008.06.010"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2009.07.006"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2007.03.010"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80306-8"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M510682200"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbin.10651","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.10651","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T23:15:20Z","timestamp":1696634120000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.10651"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8,9]]},"references-count":18,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2016,10]]}},"alternative-id":["10.1002\/cbin.10651"],"URL":"https:\/\/doi.org\/10.1002\/cbin.10651","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8,9]]},"assertion":[{"value":"2016-05-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-06-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-08-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}