{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T21:49:14Z","timestamp":1759960154096,"version":"3.41.0"},"reference-count":53,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,12,19]],"date-time":"2016-12-19T00:00:00Z","timestamp":1482105600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81471445","31271247","31071019"],"award-info":[{"award-number":["81471445","31271247","31071019"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>The aim of this study is to investigate the functional role of G protein\u2010coupled receptor 30 (GPR30) in the epididymis. We found that GPR30 is expressed in the epithelium of the immature rat epididymis and is involved in chloride secretion into the caudal epididymis lumen. The short\u2010circuit current (Isc) experiments showed that in primary cultured caudal epididymis epithelium, activation of GPR30 by its specific agonist G1 induced a mono\u2010phasic current increase, and G15, the specific antagonist of GPR30, could completely inhibit the current induced by G1. The G1\u2010induced Isc was largely blocked by application of the non\u2010specific chloride channel inhibitor diphenylamine\u2010dicarboxylic acid (DPC), or by the cystic fibrosis transmembrane conductance regulator (CFTR) inhibitor CFTR<jats:sub>inh\u2010172<\/jats:sub>, suggesting that the current was mainly mediated through CFTR. In addition, after stimulating GPR30 by G1, the intracellular concentration of cAMP in the epithelium was significantly increased, indicating that the cAMP signal pathway is involved and could be responsible for the CFTR activation. Finally, to further investigate the function of GPR30 in vivo, G15 was administrated into rats subcutaneously. The osmotic pressure of the micro perfusion solution from epididymis was measured and the sperms were collected. Results showed that there was an osmotic pressure increase of the perfusion solution from G15 treated rats. When the GPR30 was inhibited by G15 endogenously, the motility of sperms decreased. Our data demonstrated that GPR30 is involved in the formation of caudal epididymis fluid micro\u2010environment thus affecting sperm motility.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10709","type":"journal-article","created":{"date-parts":[[2016,11,26]],"date-time":"2016-11-26T05:54:17Z","timestamp":1480139657000},"page":"134-146","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Functional expression of G protein\u2010coupled receptor 30 in immature rat epididymal epithelium"],"prefix":"10.1002","volume":"41","author":[{"given":"Xiaonian","family":"Cao","sequence":"first","affiliation":[{"name":"School of Life Sciences Sun Yat\u2010Sen University Guangzhou 510275 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiehong","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Sun Yat\u2010Sen University Guangzhou 510275 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geng","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Sun Yat\u2010Sen University Guangzhou 510275 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wulin","family":"Zuo","sequence":"additional","affiliation":[{"name":"School of Life Sciences Sun Yat\u2010Sen University Guangzhou 510275 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chongfeng","family":"Lan","sequence":"additional","affiliation":[{"name":"School of Life Sciences Sun Yat\u2010Sen University Guangzhou 510275 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Life Sciences Sun Yat\u2010Sen University Guangzhou 510275 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dengliang","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Sun Yat\u2010Sen University Guangzhou 510275 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongdong","family":"Gao","sequence":"additional","affiliation":[{"name":"School of Life Sciences Sun Yat\u2010Sen University Guangzhou 510275 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher H.K.","family":"Cheng","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences the Chinese University of Hong Kong Shatin, N.T. Hong Kong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen\u2010Liang","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Life Sciences Sun Yat\u2010Sen University Guangzhou 510275 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,12,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvs016"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0052357"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00233546"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2010.20"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deq094"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2010.01.035"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2010.01100.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.024257"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.0047m.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1986.sp016222"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.168"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.106.052316"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.108.068346"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.21980"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-1605"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend.16.1.0758"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2011.07.009"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2010-0023"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2009.01148.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/37352"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.110.012872"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-012-1301-3"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1159\/000354252"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1983.tb02366.x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/2.2.87"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gaq043"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2009.12.006"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod65.5.1534"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod63.6.1873"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1992.sp019384"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2012.04.007"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2010-0979"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1042\/BC20040078"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.108.071175"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2008-0269"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-11-59"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-9-135"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.111.096891"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2004.tb02787.x"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-012-0770-y"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2006.01.016"},{"key":"e_1_2_8_43_1","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/S0022-3565(24)38887-1","article-title":"Estrogen inhibition of cystic fibrosis transmembrane conductance regulator\u2010mediated chloride secretion","volume":"295","author":"Singh AK","year":"2000","journal-title":"J Pharm Exp Ther"},{"key":"e_1_2_8_44_1","first-page":"G747","article-title":"Progesterone and estradiol inhibit CFTR\u2010mediated ion transport by pancreatic epithelial cells","volume":"271","author":"Sweezey NB","year":"1996","journal-title":"Am J Physiol"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0092425"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.22005"},{"key":"e_1_2_8_47_1","first-page":"390","article-title":"The putative G\u2010protein coupled estrogen receptor agonist G\u20101 suppresses proliferation of ovarian and breast cancer cells in a GPER\u2010independent manner","volume":"4","author":"Wang C","year":"2012","journal-title":"Am J Transl Res"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-0727"},{"key":"e_1_2_8_49_1","first-page":"504","article-title":"Nuclear estrogen receptor\u2010mediated Notch signaling and GPR30\u2010mediated PI3K\/AKT signaling in the regulation of endometrial cancer cell proliferation","volume":"27","author":"Wei YA","year":"2012","journal-title":"Oncol Rep"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod38.5.969"},{"key":"e_1_2_8_51_1","first-page":"F121","article-title":"Mechanism of adrenergic stimulation of anion secretion in cultured rat epididymal epithelium","volume":"254","author":"Wong PY","year":"1988","journal-title":"Am J Physiol"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1978.sp012174"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2012.10.037"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.241245898"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbin.10709","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.10709","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T21:48:52Z","timestamp":1749764932000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.10709"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12,19]]},"references-count":53,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,2]]}},"alternative-id":["10.1002\/cbin.10709"],"URL":"https:\/\/doi.org\/10.1002\/cbin.10709","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"type":"print","value":"1065-6995"},{"type":"electronic","value":"1095-8355"}],"subject":[],"published":{"date-parts":[[2016,12,19]]},"assertion":[{"value":"2016-07-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-11-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-12-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}