{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:21:26Z","timestamp":1760955686389},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,2,27]],"date-time":"2017-02-27T00:00:00Z","timestamp":1488153600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label \/><jats:p>Lipid rafts are an initiation site for many different signals. Recently, we reported that an EGF domain in activated coagulation factor IX (EGF\u2010F9) increases lipid raft formation and accelerates cell migration. However, the detailed mechanism is not well understood. This study aimed to evaluate the effects of EGF\u2010F9 on the cell membrane. A431 cells (derived from human squamous cell carcinoma) were treated with recombinant EGF\u2010F9. Cells were immunocytochemically stained with probes for lipid rafts or phosphatidylserine (PS). After 3\u2009min of treatment with EGF\u2010F9, cholera toxin subunit B (CTxB) binding domains emerged at the adhesive tips of filopodia. Subsequently, CTxB staining was observed on the filopodial shaft. Finally, large clusters of CTxB domains were observed at the edge of cell bodies. Markers for lipid rafts, such as caveolin\u20101 and a GPI anchored protein, co\u2010localized with CTxB. Staining with annexin V and XII revealed that PS was exposed at the tips of filopodia, translocated on filopodial shafts, and co\u2010localized with CTxB at the rafts. Immunocytochemistry showed that scramblase\u20101 protein was present at the filopodial tips. Our data indicates that EGF\u2010F9 accelerates PS exposure around the filopodial adhesion complex and induces clustering of lipid rafts in the cell body. PS exposure is thought to occur on cells undergoing apoptosis. Further study of the function of the EGF\u2010F9 motif in mediating signal transduction is necessary because it is shared by a number of proteins.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10733","type":"journal-article","created":{"date-parts":[[2017,2,2]],"date-time":"2017-02-02T10:27:32Z","timestamp":1486031252000},"page":"374-383","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["EGF domain of coagulation factor IX is conducive to exposure of phosphatidylserine"],"prefix":"10.1002","volume":"41","author":[{"given":"Chiaki","family":"Hidai","sequence":"first","affiliation":[{"name":"Division of Physiology Department of Biomedical Sciences Nihon University School of Medicine 30\u20101 Oyaguchikami\u2010cho, Itabashi\u2010ku Tokyo 173\u20108610 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuke","family":"Fujiwara","sequence":"additional","affiliation":[{"name":"Division of Physiology Department of Biomedical Sciences Nihon University School of Medicine 30\u20101 Oyaguchikami\u2010cho, Itabashi\u2010ku Tokyo 173\u20108610 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinichiro","family":"Kokubun","sequence":"additional","affiliation":[{"name":"Division of Physiology Department of Biomedical Sciences Nihon University School of Medicine 30\u20101 Oyaguchikami\u2010cho, Itabashi\u2010ku Tokyo 173\u20108610 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisataka","family":"Kitano","sequence":"additional","affiliation":[{"name":"Division of Physiology Department of Biomedical Sciences Nihon University School of Medicine 30\u20101 Oyaguchikami\u2010cho, Itabashi\u2010ku Tokyo 173\u20108610 Japan"},{"name":"Division of Oral Surgery Department of Otorhinolaryngology Head and Neck Surgery Nihon University School of Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,2,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2004.02.001"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2010.03.020"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R200019-JLR200"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2736(02)00476-5"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-05-643403"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.11.042"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00858.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12033-008-9038-7"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.10491"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2014.11.036"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2009.01.009"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00561"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2005.05.004"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biophys.093008.131234"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt929"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200503140"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M707890200"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2013.115"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-10-3195"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12325-014-0165-1"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200302028"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1247\/csf.28.419"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0156557"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.E600002-JLR200"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(98)00006-X"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2015.08.003"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.141"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/42408"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.175.4023.720"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M710386200"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09583"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1028"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114.20.3631"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-014-1294-y"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.086967"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-005-5099-y"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12944-016-0334-0"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.06.037"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V89.4.1121"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbin.10733","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.10733","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T12:11:26Z","timestamp":1696594286000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.10733"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,27]]},"references-count":39,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2017,4]]}},"alternative-id":["10.1002\/cbin.10733"],"URL":"https:\/\/doi.org\/10.1002\/cbin.10733","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,27]]},"assertion":[{"value":"2016-08-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-01-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-02-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}