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It is expressed at higher levels primarily in liver and intestine as compared to the levels in several other organs. It is activated by a broad spectrum of xenobiotics and endobiotics. The primary function of PXR is to regulate the expression of drug metabolizing enzymes and transporters and prevent the accumulation of toxic chemicals in the body, thereby maintaining body's homeostasis. In this study, we identified a C\/T single nucleotide polymorphism at position \u2212831 from the transcriptional start site of the PXR gene promoter and examined the functional significance of this variant using both the luciferase reporter gene assays and electrophoretic mobility shift assays (EMSA). Transient transfection experiments showed that the T\u2010allele was associated with significantly greater transcriptional activity than the C\u2010allele of SNP rs3814055. These results indicate that the \u2212831C\/T polymorphism has a direct effect on transcriptional regulation of PXR gene. 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