{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T11:41:36Z","timestamp":1788003696152,"version":"build-2784847793"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,3,20]],"date-time":"2017-03-20T00:00:00Z","timestamp":1489968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002632","name":"Hallym University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002632","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cell Biology International"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Human islet amyloid polypeptide (hIAPP), a major constituent of islet amyloid deposits, induces pancreatic \u03b2\u2010cell apoptosis and eventually contributes to \u03b2\u2010cell deficit in patients with type 2 diabetes mellitus (T2DM). In this study, Tat\u2010mediated transduction of biliverdin reductase A (BLVRA) was investigated in INS\u20101 cells to examine whether exogenous supplementation of BLVRA prevented hIAPP\u2010induced apoptosis and dysfunction in insulin secretion in \u03b2\u2010cells. Tat\u2010BLVRA fusion protein was efficiently delivered into INS\u20101 cells in a time\u2010 and dose\u2010dependent manner. Exposure of cells to hIAPP induced apoptotic cell death, which was dose\u2010dependently inhibited by pre\u2010treatment with Tat\u2010BLVRA for 1\u2009h. Transduced Tat\u2010BLVRA reduced hIAPP\u2010evoked generation of reactive oxygen species, a crucial mediator of \u03b2\u2010cell destruction. Immunoblot analysis showed that Tat\u2010BLVRA suppressed hIAPP\u2010induced increase in the levels of proteins involved in endoplasmic reticulum (ER) stress and apoptosis signaling. Transduced Tat\u2010BLVRA also recovered hIAPP\u2010induced dysfunction in basal and glucose\u2010stimulated insulin secretions. These results suggested that transduced Tat\u2010BLVRA enhanced the tolerance of \u03b2\u2010cells against IAPP\u2010induced cytotoxicity by alleviating oxidative stress and ER stress. Therefore, Tat\u2010mediated transduction of BLVRA may provide a potential tool to ameliorate \u03b2\u2010cell deficit in pancreas with T2DM.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cbin.10750","type":"journal-article","created":{"date-parts":[[2017,2,15]],"date-time":"2017-02-15T13:19:09Z","timestamp":1487164749000},"page":"514-524","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Tat\u2010biliverdin reductase A protects <scp>INS\u20101<\/scp> cells from human islet amyloid polypeptide\u2010induced cytotoxicity by alleviating oxidative stress and <scp>ER<\/scp> stress"],"prefix":"10.1002","volume":"41","author":[{"given":"Su Jin","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Physiology College of Medicine Hallym University Chuncheon  24252 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyung Kyung","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Physiology College of Medicine Hallym University Chuncheon  24252 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won Sik","family":"Eum","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science and Research Institute of Bioscience and Biotechnology Hallym University Chuncheon 24252 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinseu","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science and Research Institute of Bioscience and Biotechnology Hallym University Chuncheon 24252 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soo Young","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science and Research Institute of Bioscience and Biotechnology Hallym University Chuncheon 24252 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyeok Yil","family":"Kwon","sequence":"additional","affiliation":[{"name":"Department of Physiology College of Medicine Hallym University Chuncheon  24252 Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,3,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2012.67"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.252626999"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.21.122303.120200"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.52.1.102"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2004.04.036"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-1326.2010.01238.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2014.08.011"},{"key":"e_1_2_8_9_1","first-page":"38","article-title":"Biliverdin reductase: new features of an old enzyme and its potential therapeutic significance","volume":"60","author":"Florczyk UM","year":"2008","journal-title":"Phamacol Rep"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2010.09.003"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.51.2007.S455"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00404025"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00318.2007"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.2337\/db07-0197"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.110317"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2004-0405"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2014.09.003"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4781(96)00099-1"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2006.05367.x"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-006-0590-z"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.04.076"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2010.12.008"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2005.07.021"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M413121200"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/47513"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2015.02.001"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1103160"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2012.06826.x"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.2337\/db06-0734"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.113.6.1776"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20080018"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2012.00055"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0958-1669(02)00284-7"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(94)90140-6"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M308244200"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.2337\/db07-0849"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/dmrr.1140"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-008-1255-x"}],"container-title":["Cell Biology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbin.10750","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbin.10750","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T06:42:53Z","timestamp":1696574573000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cbin.10750"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,20]]},"references-count":37,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,5]]}},"alternative-id":["10.1002\/cbin.10750"],"URL":"https:\/\/doi.org\/10.1002\/cbin.10750","archive":["Portico"],"relation":{},"ISSN":["1065-6995","1095-8355"],"issn-type":[{"value":"1065-6995","type":"print"},{"value":"1095-8355","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3,20]]},"assertion":[{"value":"2016-10-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-02-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-03-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}